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作 者 ：陈金金;彭俊华

期 刊 ：植物科学学报 2011年 29卷 6期 页码：696-703

关键词：小麦；EST-SSR分子标记；可转移性；禾本科能源植物；

Keywords:Wheat, EST-SSR molecular markers, Transferability, Gramineae energy plant,

摘 要 ：为了筛选出一些有用可靠的分子标记,应用于禾本科能源植物的遗传进化研究及种质资源评价,检测了48对源于小麦(Triticum aestivum)EST序列的SSR引物对7种有潜力的禾本科能源植物:斑茅(Saccharum arundinaceum)、中国芒(Miscanthus sinensis)、五节芒(M.floridulus)、河八王(Narenga porphyrocoma)、南荻(Triarrhena lutarioriparia)、菅(Themeda villosa)和香根草(Vetiveria zizanioides)的可转移性。结果表明,在48对小麦EST-SSR引物中,36对具可转移性,可转移率为75.0%。这些可转移引物在7个物种中共检测出240个位点,其中多态性位点为184个,多态位点百分率高达76.7%。每个位点的PIC变幅为0～0.370,平均为0.220。利用EST-SSR数据构建了所研究的7种禾本科植物的系统发育树,其中斑茅、河八王、南荻及菅被聚为A组,中国芒和五节芒聚为B组,香根草单独聚为C组,基本与形态学分类一致。因此,小麦EST-SSR分子标记在禾本科近源物种中的可转移性较高,对禾本科能源植物的遗传育种研究具有重要的参考价值。

Abstract:To select some useful and reliable molecular markers for genetic and evolutionary study and germplasm evaluation of potential energy grass plants,the transferability of 48 wheat EST-SSR markers to seven grass species,Saccharum arundinaceum,Miscanthus sinensis,Miscanthus floridulus,Saccharum arundinaceum,Triarrhena lutarioriparia,Themeda villosa,and Vetiveria zizanioides were assessed.The results showed that 36 of 48 wheat EST-SSR markers could be transferred to the potential energy plants,with a transferability rate of 75.0%.Two hundred and forty loci were detected in the seven species,and the polymorphic loci were 184,with the polymorphic rate as 76.67%.The polymorphism information content(PIC)ranged from 0 to 0.370,and 0.220 was the average.A dendrogram was constructed based on genetic distance generated by the transferable EST-SSR markers.The S.arundinaceum,N.porphyrocoma,M.floridulus and T.villosa were classified into group A,M.sinensis and M.floridulus were assigned to group B,and V.zizanioides belonged to group C.This result is consistent with classical classification based on morphological traits.Therefore,wheat EST-SSR markers had high transferability to the Gramineae species,and will be useful in genetic and breeding research on potential Gramineae energy plants.

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