摘 要 ：采用一种改进的方法，从商陆属两种植物—商陆(Phytolacca acinosa)和美州商陆(P．americana)种子中分别制备抗病毒蛋白(Pokeweed antiviral protein from seeds，简称PAP－S)。研究证明，两种植物中的PAP－s性质完全相同。经SDS－PAGE和凝胶过滤层析测得该蛋白质为单一肽链，分子量约30kD；聚丙烯酰胺等电聚焦电泳测得等电点为8.4；经Edman降解，测得N-末端氨基酸为Ile.PAP－s在免网织红细胞裂解液中抑制蛋白质合成ID50为2.6ng/ml(8.6×10－11 mo1/1; 将该蛋白质与脊髓灰质炎III型病毒混合接种于猴肾细胞培养液,显示出较强的抗病毒活性，浓度为4.17×10－8mol/l时能抑制98％的病毒增殖。将PAP－s通过异型双功能连接剂SPDP与抗人T细胞单克隆抗体Wu71偶联制备兔疫毒素，对T淋巴细胞白血病CEM细胞显示特异杀伤作用，在10－9 mo1/1时可杀灭76.4％的靶细胞，14C-亮氨酸掺入蛋白质合成抑制试验显示ID50为10-10-10－9 mo1/1.
Abstract:Abstract By an improved procedure, pokeweed antiviral protein (PAP-s) has been preoared from seeds of Phytolacca arnerzcona and P.acinosa respectively. Our experiment demonstrated that the characters of PAP-s from both two species were all the same. The protein is a single peptide chain with MW 30,000 and pI 8. 4 determined by SDS-PAGE and eictrofocusing on Ampholine/polycrylamide-gel plates. Edman degradation showed that N-terminal residue of PAP-s was lie. PAP-inhibits protein synthesis in a rabbit reticulocyte lysate with an ID50 of 2.6 ng/ml (8.6xl0^11 mol/l). The multiplication of poliomyclity virus type III m monkey kidney cells infected m the presence of the protein is strongly inhibited, a 98% inhibition being caused by it at a concentration of 4.17 ×lO^-8 mol/1. PAP-s has been conjugated to monoclonal antibody Wu71 to from immunotoxin specifically toxic to T-lymphocyte leukemia CEM cells.