---

期 刊 ：植物遗传资源学报 2016年 17卷 3期

关键词：棉花；bZIP转录因子；非生物胁迫；RT-PCR；表达分析；

Keywords:cotton, bZIP transcription factor, abiotic stress, RT-PCR, expression,

摘 要 ：低温、干旱和高盐是影响棉花生长发育和产量的重要限制因素。bZIP转录因子在植物非生物胁迫反应中起重要作用。本研究利用生物信息学的方法从陆地棉中鉴定了24个bZIP转录因子基因，命名为GhbZIP1 -GhbZIP24。系统进化树分析表明，这24个家族成员主要聚集在A、B、C、D、E、G、I、S这8类亚家族。通过RT-PCR的方法分析了棉花（Gossypium hirsutum L.）GhbZIPs基因在高盐（200 mmol.L-1NaCl） 、干旱、4℃低温等非生物胁迫处理下的表达模式。结果表明，19个基因响应高盐胁迫、11个基因对干旱胁迫有应答反应，15个基因有冷胁迫应答。此外，有4个基因（GhbZIP4、GhbZIP7、GhbZIP21和GhbZIP23）在3种处理下均有应答反应。以上研究结果表明，GhbZIPs在陆地棉的非生物胁迫适应过程中可能具有重要的作用。本研究为进一步探索棉花bZIP转录因子在棉花抗逆反应中的重要作用和利用基因操作手段提高棉花抗逆性提供重要信息。

Abstract:Low temperature, drought and salinity are major abiotic stresses that influence survival, productivity of cotton. bZIP transcription factor genes play significant roles in the response to abiotic stresses. In this study, twenty-four bZIP genes were identified in cotton (Gossypium hirsutum L.) by bioinformatic approach named as GhbZIP1-GhbZIP24. Phylogenetic analysis revealed that the isolated cotton bZIPs genes can be classified into 8 groups: A, B, C, D, E, G, I, S. Semi-quantitative RT-PCR were employed to reveal the expression profiling of 24 GhbZIPs under different abiotic stresses (200 mmol.L-1 NaCl, drought, 4℃) treatments. The results showed that the expression of 19 GhbZIP genes and 11 GhbZIP genes were up- and down-regulated by salinity and drought stresses, and that of 15 GhbZIP genes were up- and down-regulated by cold treatments, respectively. Moreover, four genes including GhbZIP4, GhbZIP7, GhbZIP21 and GhbZIP23 were response to all above treatments. The results suggested that GhbZIPs may play important roles in upland cotton plant abiotic stress adaption. Taken together, our data provided valuable information for further exploring the roles of the cotton bZIPs genes in response to stress and will eventually lead to the development of genetically manipulated cotton varieties with improved stress tolerance.

---