Abstract:A new steroidal sapogenin named wattigenin- A was isolated from the fresh rhizoms of Tupistra wattii Hook.f.(Liliaceae) together with a known one, ranmognin D. On the basis of spec-tral evidence, the structure of wattigenin A was elucidated as spirost-25(27)-ene-1β,2β,3β,4β,5β,6β-hexol.