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期 刊 ：植物遗传资源学报 2015年 16卷 4期

关键词：盐胁迫；小麦代换系；渗透调节；染色体效应；

Keywords:Substitution lines, Salt stress, Osmoregulation, Chromosome effect,

摘 要 ：以中国春-Synthetic 6x小麦染色体代换系及其亲本为材料，设置对照（0mmol.L-1 NaCl）和盐胁迫（150mmol.L-1 NaCl）2个处理，通过测定不同盐处理条件下代换系幼苗渗透调节物质Na 、K 含量、可溶性糖、可溶性蛋白及脯氨酸含量变化，探讨盐胁迫对小麦代换系幼苗渗透调节物质的影响，并对其调控相关特性的基因进行染色体定位。结果表明，在盐胁迫条件下，小麦代换系的无机渗透调节物质钠、钾离子含量明显升高，有机渗透调节物质可溶性糖、可溶性蛋白及脯氨酸含量显著增加。对相关性状的染色体定位分析表明，Synthetic 6x的1A、2A、3B、7B、1D、4D和7D染色体上存在抑制钠离子含量升高的基因，4A、7A染色体上可能存在使钾离子含量升高的基因；6A、7A和7D染色体上可能存在使可溶性糖含量升高的基因，1A、2A 、4A和 6A染色体上可能存在使可溶性蛋白含量升高的基因，7A、6D染色体上可能存在使脯氨酸含量升高的基因。即Synthetic 6x的第四、七染色体（4A、4D；7A、7D）上可能含有调控无机调节物质的基因，第六染色体上（6A、6D）可能含有调控有机渗透调节物质的基因。

Abstract:Abstract: Wheat substitution lines between Chinese Spring and Synthetic 6x under the treatments of control(150mmol.L-1 NaCl) and salt stress(150mmol.L-1 NaCl)were studied to research the effect of salt stress on the changes of Na , K content, soluble sugar content, soluble protein content，proline content and to locate the genes controlling the relative characters. The results showed that contents of K and Na increased under salt treatment comparing to control. The organic osmotic substance of wheat substitution lines also increased significantly under salt tolerance. Chromosome location of relative characters showed that the genes inhibiting Na content might be located on 1A, 2A, 3B, 7B, 1D, 4D and 7D chromosome and increasing K content might be exist on 4A, 7A chromosome of Synthetic 6x, genes promoting soluble sugar content might be located on 6A, 7A, 7D chromosome and increasimg soluble protein content might be exist on 1A, 2A, 4A, 6A chromosomes and the genes promoting proline content might be located on 7A, 6D chromosome of Synthetic 6x. In sum, the fourth,seventh chromosomes (4A, 4D; 7A, 7D) Synthetic 6x may contain the genes regulated inorganic substances and the sixth chromosome (6A, 6D) may contain organic osmotic regulation genes.

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