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期 刊 ：植物遗传资源学报 2012年 13卷 2期

关键词：盐芥；ThGPX6；基因克隆；表达模式分析；

Keywords:Thellungiella halophila, ThGPX6, Gene cloning, Gene expression analysis,

摘 要 ：谷胱甘肽过氧化物酶在植物响应盐胁迫中具有重要作用。依据盐芥EST序列进行RACE实验，获得1个新的谷胱甘肽过氧化物酶基因，命名为ThGPX6（GenBank注册号为FJ357244）。该基因的cDNA全长892 bp，包含1个长为702bp的开放读码框，编码234个氨基酸。生物信息学分析表明，该蛋白具有植物GPX的典型结构，即GPX催化活性区（NVASKCGLT）和标志性基序（ILAFPCNQF），以及PHGPX特有序列（KWNF（S/T）KFL）。实时荧光定量PCR分析结果表明，ThGPX6在盐芥叶片和根中表达，其表达受NaCl诱导，显示ThGPX6在植物高盐响应中发挥作用。

Abstract:Glutathione peroxidase (GPX) plays an important role in response to salt stress in plant. Through the analysis on EST sequence of Thellungiella halophila, a novel gene exhibiting high homology with plant glutathione peroxidase was discovered and named as ThGPX6 (GenBank accession number: FJ357244)．ThGPX6 consists of 892 nucleotides，including a single 702bp opening reading frame which encodes a 234-amino acid peptide．Bioinformatics analysis showed that ThGPX6 had typical structures of GPX，such as catalysis region (NVASKCGLT), characteristic motif (ILAFPCNQF) and PHGPX special sequence (KWNF(S/T)KFL)．Quantitative real-time PCR analysis revealed that the gene expressed in shoot and root，and could be induced by NaCl in T.halophila．These results showed that the ThGPX6 played a crucial role under salt stress in T.halophila．

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