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期 刊 ：植物遗传资源学报 2012年 13卷 2期

关键词：蚕豆；种子贮藏蛋白；SDS-PAGE；亲缘关系；蛋白质亚基；

Keywords:Faba bean, Seed storage protein, SDS-PAGE, Genetic relationship, Protein subunits,

摘 要 ：以10份蚕豆品种为材料，利用SDS-PAGE方法，对其种子贮藏蛋白亚基组成进行了分析，结果表明：不同品种间的种子贮藏蛋白具有一定的差异，表现出一定的多态性；共分离出23条迁移率不同的亚基条带，13条具有多态性；利用贮藏蛋白亚基条带的信息，分析了品种间的蛋白相似度，相似度指数0.619～0.947，平均0.744；并通过聚类分析，在遗传相似系数0.8260水平上， 将供试材料分为了3类，其中第2类具有较丰富的多样性，多样性指数达3.9296；共筛选出7对亲缘关系较远的杂交组合，可为蚕豆品种选育提供一定的科学依据。

Abstract:In order to investigate the difference of seed storage proteins in Faba bean, Seed storage proteins extracted from ten species of Faba bean were analyzed with SDS-PAGE, the result showed that it is different among the seed storage proteins of faba bean, a total of 23 subunits of different Rf were detected, counting 13 polymorphic protein subunits. The similarity index of seed storage proteins subunits in Faba bean is 0.619~0.947, with an average of 0.744. Cluster analysis divided the accessions into three major clusters in the leave of 0.8260 GS, the second cluster showed higher polymorphism, and 7 pair Faba bean were identified as the parents.

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