作 者 :叶静,肖美添,昝珂,黄雅燕,张学勤
期 刊 :中国中药杂志 2016年 41卷 14期 页码:2655.
关键词:天南星科 ;千年健属 ;千年健 ;化学成分 ;抗炎 ;
Keywords:Araceae , Homalomena , Homalomena occulta , chemical constituents , anti-inflammatory ,
摘 要 :采用硅胶柱、ODS开放柱和半制备液相等色谱方法相结合,从千年健根茎乙醇提取物中的乙酸乙酯部位分离得到12个倍半萜类化合物,根据理化性质和波谱分析,化合物结构分别鉴定为3α ,7α -dihydroxy-cadin-4-ene (1 ),3-oxofabiaimbricatan (2 ),3β ,4α -dihydroxy-7-epi-eudesm-11(13)-ene(3 ),integrifonol A (4 ),1β ,6β -dihydroxy-7-epi-eudesm-11(13)-ene (5 ),4β ,7β ,11-enantioeudesmantriol (6 ),epi -guaidiol (7 ),oplopanone (8 ),(-)-1β ,4β ,6α -trihydroxy-eudesmane (9 ),2α -hydroxyhomalomenol (10 ),(-)-T-muurolol (11 ),hamalomenol A (13 )。 化合物 1~7 为首次从千年健属植物中分离得到,11~12 为首次从该植物中分离得到。此外对分离的部分化合物进行了抑制脂多糖诱导的小鼠巨噬细胞RAW 264.7细胞NO释放实验,发现化合物3,5和8有良好的抑制活性,其IC50 分别为6.51,3.25,7.78 μmol·L-1 。
Abstract:Twelve compounds were isolated from alcohol extracts of the rhizome of Homalomena occulta by using various chromatographic techniques including column chromatography onsilica gel and C18 reverse-phase silica gel, and semi-preparative HPLC. Their structures were identified by physico-chemical properties and spectroscopic data analysis as 3α , 7α -dihydroxy-cadin-4-ene (1 ), 3-oxofabiaimbricatan (2 ), 3β , 4α -dihydroxy-7-epi -eudesm-11(13)-ene (3 ), integrifonol A(4 ), 1β , 6β -dihydroxy-7-epi-eudesm-11(13)-ene (5 ), 4β , 7β , 11-enantioeudesmantriol (6 ), epi -guaidiol (7 ), oplopanone(8 ), (-)-1β , 4β , 6α -trihydroxy-eudesmane (9 ),2α -hydroxyhomalomenol(10 ), (-)-T-muurolol (11 ) and hamalomenol A(12 ). Compounds 1-7 were obtained from the genus Homalomena for the first time and 11-12 were firstly reported from the species. Additionally, compounds 3, 5 and 8 displayed inhibitory effects against the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse macrophage RAW264.7 cells with IC50 values of 6.51, 3.25, 7.78 μmol·L-1 , respectively.
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