免费文献传递   相关文献

Mechanism exploration on synthesis of secondary metabolites in Sorbus aucuparia cell cultures treated with yeast extract

酵母提取物诱导欧洲花楸悬浮细胞合成次生代谢产物的机制探究



全 文 :&()}Ž*+,-./01á›
2¹R¬‚}‡z3¥
ž
!
Ÿx-
!

!
 ¡
!
X‡œ
!
¢£?
!
Z¤¥
!

Ù‚IŽ ƒÇÈÑ ÃæƒáÙçÜÝÎϊèÛéæ
!

$$%$$#

bc
#!
2*+,-./ëì01
!838<
¾Ïé
$
^ñ&()}
!PI
dÝW
838<
¹Ý¹ÞIJ‡ÏÞ
$
-¼¥
PI
Ž
838<
á›2¹R¬‚}‡z3
&
Þù‘(
# PI
Ž
838<
á›ð

H:2šÞá}
$
d

HÞá}ðÐŽQ
ˆ‡EËñºâã‡ÏÞæç
PI
dÝY301‡¹Ë

01ëì¡$‡
]ÂadÝQˆ‡EË01Ό

01$‡Í}
¿øÓðБº¾ƒ„F·ÏÞòâ
$PI
dÝ­
!PI
­

Í}¿øÓðÐCÎ6­
!­

‚«[\ž’
$
-ËQ‚
ΞВ,ð
&%O%#j­Ñ
PI
­01]
*l
f‘º¾8S
$
¬
PI
­‡8S([:±
­
&
ç(
PI
Ž>01d±
8HØٌŠ
$
⌱01‡¹Ë
$
Ù>01á›ð:2šÞá}@Í]ûØÙ

018Í}¿øÍÅ´¾XšÇC»[
Þá}‡á›
*l
l<œõÍň01ˆÎ
PI
Øهl<\
&

def
#!
*+,-./01

&()}

2¹R¬‚}

z3

gh)(
#!*$)5*5*$

ijkP
#!
%øù&‰úû
!()$$%$$($%*4(4

&(ÛNxOúû
!*$*J3:*4J$*$*$*J3:*(J$$*

lmno
#!
!
9©„
$@FW#!$$ #&$4&&$I5/GSW# EW]$2*#O
-./

noxy
#!
®þ
$I5/GSW# R1WFS5*%2#)O-./
!!
*+,-
A+%<=#$=&=F$%/$ CO
¾ÜÝ&Þù®
&,-Áΰn³u:n³
$
B‚*+Ñ®+M1
Å:Ë*Lš
*+
$
‘Öϛ^ùzÍ2‰ÑX
,
**+
&
µ%,-ÁŠ}
$
ÃH
$
‘@Þß
$
l,‰

#
H
**+
&
û²†cº
$
Þù®&Š}Ž,]
û}Bű—QßÜ¿H‚¹:2šÞá}´¾Š

*)+
$L.KXDX1 @
á>‰ß´õdÝ*+,-°
yW
$
Í2+°y$œ´Ž*,-¯
*&+
$
ßH:2
šÞá}´«‡Š$¾âZ
$
d‘ND=´
‡¹}¾¿
&
*+,-Óº,‰
%

%
A|ѹŠ
æ÷±8X‡räH
&
*+,-./01
! 838<
¹ËYp
%
¤v
S
$
Í´¾?LJ†Ïé
*+
$
ØD†cº‰&
()}
!PI
´¾ŽõdÝÍ>
838<
áYá
›:2šÞá}
*#+
&
¬Ó
$
û²ßšNDŠj¯´
‰‡Þá}á›z3üâ(}
$
WDcž8¼‡
†
&
9z{2
838<
¾Ïé
$
Bg^ñ
PI
dÝW
838<
2¹R¬‚}‡Ïސ‘01ëì¡$
]\%
Í}¿øðÐ
%
01]
*l
Sá¹Ý¹ÞIJ‡
ÏÞ
$
-¼¥
PI
Ž
838<
á›2¹R¬‚}‡
z3
$
F‘Ó+Í}¿øÑÖ´õ
*
Ç£Q¾z
3†(lð?LJFPÑ£Ú
$
¾cõ22H†
ãFá
&
!
Ïé
*+,-./01ä“$%&lŠ}†E
°Ñ膝àá
&
ëì!<.1eSU.1 3VG]?ST<9B#$$
˜”÷Ñ
Z
!aP83$)
Wõ€e
!8GU?.USXTJ8 **$*8
˜
Ör´Ê
!8N5÷³ÿÏQÖÞr´Ê

œ
†÷>x
!YI;N3P)$
ËP¡‚5
!NG?FUT
*#4 d*44#A_
^ñ?
!@#
ÿÀÁ
45#$5$5
#4
¡Ó:‰5
!3ESWF1?#)*$ :.1 @UG]
‘‹æ
æ~Â
&
㊦ø¾£%
9B
FG‚Ò
PFGT?F7?UG-?
¾
b^^ :Z
FG‚Ò

‘‹,Íô¾%,ºíÞ,
ÍDEFG‚Ò
&
!
£¤
_!
01./ëìí䇼î
!
ŽŽ‡*+,
-`ž­€ôH±ð
$ /C98
Ïíë쏇
&$$
/C
=ŸÐ$
$
`ž­€u
& V
;R

2
$
/g
) m
2;RW
$
컧c‡`ž­€\X¡íëì$
ëì
$
u
*$ /C
NäÐ$œe
$ /C
‡¡íëì

$
±
* r
ëì\$~ëì
&
./01ëì\Y
,4$*,
t
)4
~t

v
*$&
o
#
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
_.WO)4$:TTXF!
YX1F$ *$&
~ò¾
*$ U,/S1
k
&
z{$>‰‡*+,-./
01ÓjÊÀgÝW‡
O& E
01ôH±ðD
*$
/C98
¡íë쏇
$$ /C
NäÐ$
&
_!PI
Žõ‡3dÝ
!
‰ªÔè}¡&
()}
$
3
#$ E,C
k
‡¡
$
ËÀ{Å
&
Ç6
CSX
J
ádÝ£¤
*#+
$
dÝÌ_¾
) E,C
k
&
_&!
*+,-./01$2¹R¬‚}‡()Ñ

!
jªÈ‡01‰
/C
šÞ†Ù$†Û
$
>‰Ý|ÑâãjÝ¡gÝ,AUÐ$
$
gÝW‡
01’a+‰
$ /C
šÞ()

2
$
á¾Ý¡
$
jA
UЁ±Â\ªc5M_²Ö
&
ë쏉
/C
®>®?ä)
*
2
$
‰œ¡â㜴
$
j®>®?2
ûX()01W‡AUÐ$
$
±Â\ªc5M_
²Ö
&
‰
/C
‡®>®?ò½
&
j()¡$‡®>®?}¡Ö
$
‰
$$
)
C
‡
=OšÞ}¡
$
g
$O**
)
/
‡DzÝ¢W‰±
\BC<

&
\BC<
ñò_о
#
¡‚=Oܾ
NG?FUT86/5
/F?U6<
(
! &O# //q$ //$
)
/
Sª‚¾
$Oj
š>è
5
šÞ
!$s$
SY
$O% /C,/S1
k

˜
]^ñh˾
*($ 1/&
ÓO_о
#
´õ@¾
I8:
^´õ7

åÞ
rÀ«
*$#,( KBG
ÖÏr¾Kr
$
ÖÏrō
)$$
r$
SY
$ C,/S1
k

‹04WÀ
& $$$ _
‚Îœ
õÓÐæ1qã
$$ m#$$
ÜBœS
*s&
_)!
*+,-./01¹}Ðñò
!
jëì‡.
/01Z‡çÝâèèW
$
6ôïÐ01†äx
¾¹}Ð
$
䠊
!MN
‘1
$
uÇdÝ~
&
ǹ
}äÕ
&
_$!
01./¡
]ñò
!
9‰œ†÷>x6
ôñòëì$‡
]\$
uÇdÝ~
&
ǹ}
äÕ
&
_*!
01$Í}¿øÓðЇñò
!
‰ŒTªe
É
Q5*$
¤ñò
*%+
$
2㊦ø´JV½`
$
ς¾
/E,E
k
!
†ä
$
uÇdÝ~
&
ǹ}äÕ
&
_%!
01]
*l
SÏއñò
!
9‰³_ž¯ñ
()
!;9@
*(+
$
‰ƒ\†þ>éyÏò01
$
’X
ñ,¡—ò
/S1$
[¯êF„,6h¦
*$$
)
/
‡01ë
$
ñòQˆ¾
$ /S1$
uÇdÝ~

ǹ
}äÕ
&
&!
ÞùCœ†
&_!
&()}Î*+,-./012¹R¬‚
}‡ÔÕ
!
01/
PI
dÝW
$
‘01()}
$^ñ,

H:2šÞá}
$
œ fB¾
*f5^\5
GX-X]GUS1! $)$5VSR6VU.765DS]RF16W! $ 1.UGX5
-X]GUS1!& $GX-X]GUS1!) $FUS.D.HXUG1!$ &
Âa
ŽQˆ‡EË
$838<
ᛇ:2šÞá}k
ÐñMTòâ
$
¬

H:2šÞá}01$‡
ðБœ ñºŽâã‡ÉÊæçrg
&
O*f5^\5GX-X]GUS1*O)$5VSR6VU.765DS]RF16W)O1.UGX-X]GUS1&OGX5
-X]GUS1OFUS.D.HXUG1&
g
!PI
dÝW
838<
$:2šÞá}ðЇÏÞ
MSEO!@RF-RG1EFTS1 DS]RF16WT-.1?F1?.H838PI
Þá}


PI
Ž
*& R
W£Í^ñ,
$

&(
R
QîÞá}‡ðВ,l÷
$
ÂaŽQˆ‡E
ËðÐ01ÊË
$
¬Ó
# R
Q
$
‘ðÐiñºž
’òâ
&
Þá}


PI
Ž
*& R
Q_’,l÷
$

‘‹^ñQˆ…MîÞá}‡ðÐfʌ
&
Þá}
&
Ó
PI
ŽW-t^ñ,‡Þá}
$
‘ðБºŽ¤v¿hª‡òâ
$* m)# R
‘ðÐ
[\ž’
$)# R
QŽºl÷
$)# m4# R
ðÐi[FÎ
$$( m#( R
ðÐ+2ƒ„ž’
&
Þá}
)
ðЂΑ‹Þá}ÊË
$

PI
Ž

*& R
HW‘ðÐ01ž’
$)* R
ðВ,-”
÷
$)* m#( R
‘ðÐñºƒ„FÎòâ
&
PI
dÝW^ñ,Þá}
$
‡Qˆ-U
$
6,
)# R
îÞá}’ÉÊ,8ò‡_
&
ÂadÝQ
ˆ‡EË
$
îÞá}‡ðÐñhª¿ž’‡òâ
$
#( R
Q‘ðВ,-”÷
&
“2MÞùÍ^
PI
ŽõÎ
838<
á›:2
šÞá}‡ÔÕÓ8ǪŠ‡ÏÞgö
$
ÂadÝ
Qˆ‡ÏÞ
$838<
á›âã‡:2šÞá}‡­œ
‘­œˆ‡«fc¹ð_Ï
&
,$*$*,
t
)4
~t

v
*$&
o
#
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
_.WO)4$:TTXF!
YX1F$ *$&
&_!
&()}Î*+,-./01¹}ЇÔ
Õ
!PI
Î
838<
¹}ЇÔÕrg
*
$‡zf÷
$
¾ð¡
PI
ŽW01¹}ÐC
­‡ÏÞ
$
n
ØÉ,‡
838<
¹Ë½`‡êáä?
#,!G o
$O)#4 4=* l$O%% F7]! k$O)*4 *G+
*+
&
g
*!
01¹}ЇÝ0÷
%
zf÷7>Q÷ÂQˆ‡
ÏÞ
MSEO*!@RF-RG1EFT+S?R ?S/F.H-FWDS./GTT?RF.UF?S-GWeGWXF
G1V G-?XGWeGWXFG1V ?RFVSHFUF1-FDF?+FF1 ?RF/
j)àQˆ…RXä?
$
É,ð01^æ¹
Ë_ÐF¹}ЇÝ0÷
!
g
*
$‡Ý0÷
$
‰É
,‡Ý0÷Êòz{$ñɇzf÷
$
É,ðÝ0
÷Ñzf÷Hˆ‡Q÷
!
g
*
$‡Q÷
&
Ғ
PI
ŽõW
!
;gzf÷
$
01¹}
ÐñºŽƒ„‡tž’iÊˇgö
$
‘ÏÞÿ
¾âÓ?([
&
)àQˆõ8
$
01¹}ÐÏÞ
â”
&
œ†Ý0÷
%
zf÷Hˆ‡Q÷
$
ç(
PI
‡
ÔÕF
$838<
žË½`Q´ð01žË÷‡½
`
$PI
Î
838<
‡Ô՝푺¾Y3´‰
&
Âa
PI
Î
838<
ŽQˆ‡ž’
$PI
Î
838<
‡Y3´
‰G01ž”
&
&_&!
&()}Î*+,-./0101./¡
$
]‡ÔÕ
!
ÂadÝQˆ‡ž’
$
01./¡
‡
]Gþåþ:
&
01./¡‡
]Ïޔh¾
*
ÇGà7FÎ
$
rg
)& &( R

$( R$]ƒ„F
Î
$

$( R

#O&&$
×t

GõÞì


*$ R
Q01./¡

#O$&$

*$ m#(
R$]d±Ø8ÇFÎGõ
$
¬F·ÿÊMÇG
õٔ
&
^æ01ë쌊F
$
01./¡$‡

aëìQˆ‡EËÛ01TË
*+
&
Ғ
PI
W01
./¡
]ñ([FÎòâ
$
ç(ÍÅÓ
838<
‚¹
!!
g
)!PI
dÝW
838<
01./¡$
]‡ÏÞ
MSEO)!@RF-RG1EFT.H?RF]\.H838<-FW-XW?XUF/FVSX/
?UFG?FV +S?R PI
‡2¹R¬‚}h
]ÏÞ
$
¾dßH
]‡_Ï
¾âŒ±01‡¹Ë
&
&_)!
&()}Î*+,-./01$Í}¿
øӇÔÕ
!­01$‡Í}¿øÓðБ
º¾ƒ„F·ÏÞòâ
$
rg
&&

*&$&( R

Ð-Ë
$
ÂaQˆ‡EËÍ}¿øӇðÐã6

$

*$$&&$#( R )
ÇQˆ…M
$
Í}¿ø
ӇðЂQ­W
& PI
­
838<
$Í}¿øÓ
‡ðÐñºŽƒ„FÎ
$
+ò±—ò‡òâ
& *& m
4# R
01$‡Í}¿øÓðÐ01FÎ
$4# R
H
W01$‡Í}¿øÓðÐ01ò±—ò
&
g
&!PI
Î
838<
8Í}¿øÓðЇÔÕ
!Dp$O$
MSEO&!@RFFHF-?T.HPI.1 ?RFT.WeF1?]U.?FS1 -.1?F1?S1 838<
!Dp$O$
j
PI
­01Í}¿øðÐC
­‘Q
$
É
,
PI
dÝW01$Í}¿øðЇžÐ
$
W
C
­Î«É,018Í}¿øӇ‚Ξ
Ð
$
rg
&
+g$ͦ¤ÉŽ
$PI
dÝW018
Í}¿øÓðÐC
­Î«
$
¸Ù/Ð
*
ÇG
õ
$
t

ÇGõ‘‚ΞДJ
$j
ÜÝ
$*$ R
HWžXt
*
ÇGõ
$
018Í}¿ø‚ΞÐ
’,ð
&$j
ÜÝ
&
,*$*,
t
)4
~t

v
*$&
o
#
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
_.WO)4$:TTXF!
YX1F$ *$&
g!PI
dÝF
838<
018Í}¿øÓðЇ‚Î
žÐ
MSEO!@RFUFWG?SeFS1-UF/F1?GW.HS1?UG-FWXWGUT.WeF1?]U.?FS1
-.1?F1?.H838&_$!
&()}Î*+,-./01]
* l
S
‡ÔÕ
!PI
Î01]
* l
S‡ÔÕ
$PI
­C
­01]
* l
f‘º¾8Sº…
$
¬Ó
PI
­
* l
Seô÷[\:±
­
&
+ªŠSY
g$GÍ2Ɏ
$
å
PI
­C
­fÓñ
ò-v
* l
8SYÊ”
$
ÂañòQˆ‡EË
Y01Ê:
$
¬
PI
­Y6Ì:±
­
&
ç(
PI
dÝWh01]
* l
8S”ÐÊË
$
rg
#&
3O
SYeô÷
JO
ªŠSY
&
g
#!PI
dÝF
838<
01]
*l
S‡ÏÞ
!Dp$O$
MSEO#!@RF-RG1EFTS1 F7?UG-FWXWGU*l
HWX7.H838)!
¦0
PI
dÝW
838<
()}$^ñ,

H:2š
Þá}
$
Û^æëì_ÐF01¾áDá›ßšÞ
á}
$
ç(01á›ßšÞá}åˆÎ
PI
Žõ
‡ØÙ
&
ãQ
$
ÂadÝQˆ‡EË01ëì¡$
‡
]01Ό
$
01¹}ÐC
­«žË([ƒ
„
$
ç(
838<

PI
ØÙFᛇ2¹R¬‚}Í
Å_Ïðëì¡$‡
]\$
hð01‡¹Ë_Ð.
Þ
%
ÔÕ01¢‡B°¿
$
>01âÅ^æžd¹
Ë
%
œÞ
%
ž/á¹v¾ª
$
-̏h01‡¹}Ð

&
D†fñ:2šÞá}á›gö$D8ò‡
\Þßú
*4+
$
9†cº

H:2šÞá}ðÐÂ
adÝQˆ‡EËñºâã‡ÉÊæç
$
í‘º
¾
#$$&
<Þá}dÝW
* R
x͉ºD£¤^
ñ,¬‚ÎðÐʌ
$
¾dÉÊÐáY’,l÷
WFÎ
$
fBß
)
HÞá}ÍÅÓ:2šÞá}¹
}á›ghM‡M¥Þá}u$ˆí
)$$
Þá}
ÉÊQˆ‚ÎÊí
$
dñÊ:hª‡—òÉʌŠ
$
‘ÍÅd±á›gh‡F¥
$
Þ߂Ηò
&

8òöM(}ð:2šÞá}HˆZ‡Þ
$
¾(}\Þßú(lð…n
&
PI
­01$Í}¿øӇðÐC
­Î
«ND[\¿QÜ
$

PI
ŽF
$
Í}¿øӇ
‚ÎðÐþåþ”
$
-ËQ‚ΞВ,ð
&%O%#j$
ç(01¾ð@°ZÅ:凞—
$
ÎÈ
Ôz3‡.XGþåþƒ
$
ƒá›‡Í}¿øÓ
ÍÅÓ-¹¹ËEâRه
$
‰å»ÌÉÞ2¹R
¬‚}‡á›
&
9z{†cº
PI
dÝW
838<
01]
*l
8SC
­Î«[\ÊË
$
ç(01Ž,
PI
Ž
õØÙW
$*l
´¾l<œõÍň01á›
2¹R¬‚}‡l<\
&
9z{Bgœ†¹}Ð
%
01ëì¡$‡
]2018Í}¿øðЇÏÞ
$

PI
Ž
838<
á›2¹R¬‚}‡z3CrH,ϋ›‡
,**$*,
t
)4
~t

v
*$&
o
#
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
_.WO)4$:TTXF!
YX1F$ *$&
*$+
98h
&
âŽ]ûOOØ
هOPF
$
Š}stè~‡Ó¹Ëј/

QÎ
â㐯‡ØÙQ
$
Š}+á›2¹R¬‚}@Í
]ûâŒOO
$
¾×RÙ¸Ž‚ˆ‡›9
$
Û+Î
ŒŠ}‡-¹¹ËY
&
×]
$
01ˆÎ
PI
ØÙ
áYóªð
*l
l<ϛ
$
ç(
*l
Íň
838<
á›2¹R¬‚}‡l<\
&
ßà¾
PI
Ž
838<
á›2¹R¬‚}‡z3†êŸðF
P
$
¾F8¼õ22‡†ãFðvzá
&

pqrs
#
* +!
$%&l$%Š}./0Ñ+
,
$%Š}.
,
t
)#
~
*9+ ,
23
#
&(Žbc
$ 4%&# *(&,
* * +!
v$U
$
dë
,
3R,‰Š}ú
,
t
)
ð
*9+,
23
#
Àû
ghŽbc
$ *$$(# &)#,
* ) +!L.KXDX1 @$ \GUD.U1FYJ,BR6?.GWF7S1 S1VX-?S.1 S1 ?RFTG]+..V
.H]WG1?T.H?RF9GW.SVFGF!>.TG-FGF DS]RF16WT.UVSDF1[.HXUG1T
*Y+,BR6?.-RF/ST?U6$ 44$ &$!###&4,
* & +!L.KXDX1 @$ \GUD.U1FYJ,3TXUeF6.H]R6?.GWF7S1 S1VX-?S.1 S1
WFGeFT.H?RF>.TG-FGFD6-.]]FUS.1T*Y+,a;G?XUH.UT-R$ 44&$
&4!<##*(,
* +!
dï“
$

$
9©„
$
á
,
*+,-01./ëì¹Ëª
«†
*Y+,
$%ºR$,-.
$ *$)$ !%##4,
* # +!CSX J$ JFXFUWF@$ JFFURXFTC$ F?GW,JS]RF16WT61?RGTFHU./
6FGT?5F7?UG-?5?UFG?FV -FW-XW?XUFT.HA+%<=#$=&=F$%/$*Y+,BWG15
?G$ *$$&$ *(!)#&4*,
* % +!

$
ï
$
óz(
$
á
,
ŒTªeÉ
Q5*$
1=¤ñò
M§$Í}¿øÓðÐ
*Y+,
9ýþ”a
#
ù&
b
$ *$$#$ !)#*),
* ( +!
_ð„
$
MW%
$
ýþ
,
³_ž¿æ1´õì»W:()
‘ËáŠ}†$‡ˆ‰
*Y+,
ù&žŸ
$ *$$#$ #
!)#*#*,
* 4 +!\w?1FU<$ JFXFUWF@$ JFFURXFTC$ F?GW,ZSHFUF1?SGWFHF-?.H
FWS-S?.UT.1 DS]RF16WG1V VSDF1[.HXUG1 H.U/G?S.1 S1 A+%<=#$=&=2
F$%/$ -FW-XW?XUFT*Y+,Y3EUS-M..V 4%%,
*$+!
®sŠ
$
9©„
,
OOØÙF2¹R¬‚}‡ÉʐÎrH
,ϋ›‡ÔÕ
*Y+,
$%$,-.
$ *$$%$ )*!&#*%%O
=@SD.B/J-@OL0AF.C/ABABJEBCD@J/JAKJ@SABG.FE -@C.MA0/C@J/B
/,07$%$&$10* S@0SH0CHF@JCF@.C@GX/CDE@.JC@OCF.SC
\A3;QCFS$ b:3^ NF150XG1$ P3;QQXG1E$ 9^ QF$ C:;8RX5HG1E$ NAaRS5EG1E$ QA^ CG15]S1E
!
!A*$*B:N$<+%$*+%:+,@$+2./P%<#$ K$*/+)$0!#+=%&()*%+,(/)#1$*%/$ 1./&$$
(/)$ ;&$.3:+,(/)#1./&$0A&/)&#$ 6/7/)5 $$%$$$ (/)$
*5MJCF.SC+!8XT]F1TS.1 -XW?XUFT-FW.H8.UDXTGX-X]GUSG!838< +GTXTFV GT/G?FUSGWT$ ?RF-RG1EFT.H]R6TS.W.ES-GWG1V DS.5
-RF/S-GWS1VF7FT.H838S1 838T61?RFTS[FHSeFDS]RF16W-./].X1VT$ G1V ?RFTF-./].X1VT-.1?F1?-RG1EFV VSHFUF1?W6+S?R S1VX-?S.1 ?S/F]U.W.1ES1E PI?UFG?/F1?S15
RSDS?FV -FWEU.+?R$ ?RF-XW?XUF/FVSX/]\+GTEUGVXGW6UFVX-FV GH?FU?UFG?/F1? +G?FU5T.WXDWF]U.?FS1 -.1?F1?TR.+FV G?UF1V .HTW.+
VF-WS1F$ +RS-R +GTTSE1SHS-G1?W6S1-UFGTFV S1 PI?UFG?/F1?EU.X] !PIEU.X] -./]GUFV +S?R ?RF-.1?U.WEU.X] !/X/UFWG?SeF-.1?F1?+GT&%,%#j S1 -.1?UGT?+S?R *l
HW.+S1HWX7$ DX?
?RFPIEU.X] HW.++GTTSE1SHS-G1?W6TW.+?RG1 -.1VX-SeF?.?RFEU.+?R .H-FWTG1V H.U-FV ?RF-FWT?.T61?RFTS[FDS]RF16W-./].X1VTGEGS1T?F7?FU1GWT?UFTT+G?FU5T.WXDWF]U.?FS1 /G6
TFUeFGTS1?UG-FWXWGUF1[6/FTS1e.WeFV S1 ?RFT61?RFTST.H-./].X1VTUFEXWG?S.1*l
/G6GTTSE1GW/.WF-XWF/FVSG?F-FWTSE1GW?UG1T5
VX-?S.1 UFT].1V ?.PIT?UFTT,
*]@E XAFGJ+!TXT]F1TS.1 -XW?XUFT-FW.HA+%<=#$=&=F$%/$ 6FGT?F7?UG-? TF-.1VGU6/F?GD.WS?FT /F-RG1ST/
GA/#([)*bPSWS--())

tu-0
!
z{|
#
,)*$*,
t
)4
~t

v
*$&
o
#
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
_.WO)4$:TTXF!
YX1F$ *$&