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期 刊 ：中国中药杂志 2007年 32卷 12期 页码：1171.

关键词：金荞麦饮片；高效液相色谱-电化学检测；含量测定；指纹图谱；

Keywords:Rhizoma Fagopyri Dibotoryis, HPLC-electrochemical detection, determination, fingerprint,

摘 要 ：目的：研究金荞麦饮片高效液相色谱指纹图谱及含量测定方法。方法：采用高效液相色谱-电化学检测法,Diamonsil C₁₈(4.6 mm×250 mm,5 μm)色谱柱；流动相为甲醇-0.1 mol·L^-1磷酸盐缓冲液(pH 2.5)；流速1.0 mL·min^-1；柱温35 ℃；参比电极为ISAAC(in-situ Ag/AgCl)；工作电极为玻碳电极；辅助电极为铂电极。结果：建立了金荞麦饮片高效液相色谱图谱,发现了6个色谱峰为共有峰,其中4个分别为没食子酸,原儿茶酸,原儿茶醛和表儿茶素的色谱峰。原儿茶酸的质量分数为0.004%～0.05%,原儿茶醛的质量分数为0.003%～0.015%。结论：该方法可用于金荞麦饮片质量控制。

Abstract:Objective: To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis. Method: The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C₁₈ (4.6 mm×250 mm, 5 μm) which was eluted with methanol-0.1 mol·L^-1 phosphate buffer (pH 2.5), the flow rate was 1.0 mL·min^-1, the column temperature was 35 ℃, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun. Result: The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and (-)-epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively. Conclusion: The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

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