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期 刊 ：中国中药杂志 2003年 28卷 07期 页码：629.

关键词：药对；溶出率；黄连；吴茱萸；

Keywords:couple of Traditional Chinese Medicine, solubility, Rhizoma Coptidis, Fructus Evodiae,

摘 要 ：目的:考察黄连与吴茱萸按不同比例配伍时,黄连中主要化学组分溶出率的变化规律。方法:黄连与吴茱萸分别按1:0,6:1,3:1,2:1,1:1,1:2,1:3,1:6,0:1等9个比例配伍,水煎,制得各供试样品,在优化的RP-HPLC条件下进行分析,得到色谱分析数据,然后考察其中8种组分的溶出率与配伍比例之间的关系,建立回归方程。结果:8种组分中,1种组分的溶出率保持相对稳定;6种组分与黄连比例成线性关系;另外,检出1种新组分,它与黄连比例成非线性关系。结论:黄连与吴茱萸配伍后各种黄连生物碱的溶出率成相同比例下降,并检出1个二药单煎时未能检出的组分。

Abstract:Objective: To investigate the relationship between the solubility of Rhizoma Coptidis‘ main chemical constituents and the proportion of Rhizoma Coptidis and Fructus Evodiae. Method: 9 samples were selected in which the proportion of Rhizoma Coptidis and Fructus Evodiae were 1:0,6:1,3:1,2:1,1:1,1:2,1:3, 1:6 and 0:1, respectively. The samples‘ water-extracts were analyzed by RP-HPLC. Then solubility changes of eight chemical constituents were calculated, and the regression equations were established. Result: Among the eight chemical constituents,one was independent on the change of proportion of Rhizoma Coptidis and Fructus Evodiae, six having linear relationship. A new constituent was detected, and there was nonlinear relationship between its quantity and the proportion of the couple. Conclusion: The solubility of Rhizoma Coptidis‘ main chemical constituents declines almost in the same scale when Rhizoma Coptidis and Fructus Evodiae are used as a couple, and a new constituent has been detected which can‘t be found in the mono-extraction.

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