Abstract:Crystals Ⅰ~Ⅳ were isolated from the ethereal extracts of Poria cocos and identified respectively as O-acetyl-pachymic acid (Ⅰ), 3β-hydroxy-lanosta-7,9 (11), 24-trien-21-oic acid (Ⅱ), β-amyrin acetate (Ⅲ) and 3β-hydroxy-16α-acetoxy-lanosta-7,9 (11), 24-trien-21-oic acid (Ⅳ). Crystal Ⅲ was obtained from P. cocos for the first time, and crystal Ⅳ was newly discovered.