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作 者 ：牛泱平,邱燕燕,竺佳,姚炳兴

期 刊 ：中草药 2009年

关键词：七叶皂苷钠；小鼠急性淋巴白血病细胞L1210；增殖；白血病；

Keywords:aescin(sodium aescinate), L1210 murine acute lymphoid leukemic cell line, cell prolifera-tion, leukemic transplant model,

摘 要 ：目的探讨七叶皂苷钠对小鼠急性淋巴白血病L1210细胞的体外增殖抑制作用和体内抗白血病作用。方法采用MTT法观察七叶皂苷钠对小鼠急性淋巴白血病细胞L1210的体外增殖抑制作用;建立L1210小鼠白血病移植模型,观察七叶皂苷钠对荷瘤小鼠生命的延长率,以及对化疗药阿霉素的增敏作用,并绘制生存曲线。结果七叶皂苷钠（20～60μg/mL）对L1210细胞具有一定的增殖抑制作用,作用呈时间和剂量依赖性,体外作用72 h的IC₅₀为（24.86±2.23）μg/mL;体内实验结果显示,七叶皂苷钠中、高剂量组能明显延长荷瘤小鼠的生命期,生命延长率分别为32.3%和25.8%（P<0.01）,低剂量组对常规化疗药阿霉素具有增敏作用。结论七叶皂苷钠能有效抑制L1210细胞体外增殖,对体内L1210白血病小鼠移植模型也具有一定的治疗效果。

Abstract:ObjectiveTo explore aescin‘ s inhibition of in vitro proliferatim in L1210 murine acute lymphoid leukemia cells and its in vivo antileukemic effects.Methods MTT assay was used to determine the anti-proliferative effect in vitro;Leukemia transplant models were used to test the antileukemic effect.Mice inoculated with L1210 cells were treated with ten daily doses of aescin or/and each other day of DOX，and were observed for survival.Antileukemic effects were assessed by calculating the extension of lifespan.Results Aescin (20一60 p.g/mL) was found to be able to inhibit the proliferation of L1210 cells. The effects were in a dose-and time-dependent manner.The IC₅₀, value of aescin in L1210 cells after 72h was (24.86±2.23) ug/mL.In vivo study showed that after treating the L1210 cells bearing mice with higher,middle,and low dosage(4:5,3.5，and 2.5 mg/kg) of aescin for ten consecutive days，The extension of lifespan were 25. 8%(P<0.O1)，32.3%(P<0.0l)，and 12.9%，respectively.Moreover, the extension of lifespan was 45.2%when mice were treated with a low dose of aescin in combination with DOX(2mg/kg)，which was significantly increased compared with 29.0% when treated with singleDOX (P<0.01). Conclusion The data presented here indicate that aescm is a poent natural inhibitor of proliferation in L1210 lymphoid leukemia cells and aesctn has the potential to be a promising candidate for cancer treatment.

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