全 文 ： 2011 年 3 月 第 9 卷 第 2 期 Chin J Nat Med Mar. 2011 Vol. 9 No. 2 141

Chinese Journal of Natural Medicines 2011, 9(2): 0141−0145
doi: 10.3724/SP.J.1009.2011.00141
Chinese
Journal of
Natural
Medicines







In vivo Antithrombotic and Antiplatelet Activities of a
Quantified Acanthopanax sessiliflorus Fruit Extract
SONG Yang, YANG Chun-Juan, YU Kai, LI Fa-Mei*
Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China
Available online Mar. 2011
[ABSTRACT] AIM: To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol
extract of Acanthopanax sessiliflorus fruits (ASFEtOH). METHODS: In the present work a quantified ASFEtOH was selected to deter-
mine its in vivo antithrombotic activity in a rat arteriovenous shunt thrombosis model and its inhibitory effect on adenosine diphosphate
(ADP)-induced platelet aggregation using a platelet aggregometer. Ten triterpenoid saponin contents of the extract of the fruits were
determined by a validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Total triter-
penoid saponin content was also determined according to the colorimetric method by ultraviolet-visible spectrophotometer. RESULTS:
After ASFEtOH (125, 250, 500 and 1 000 mg⋅kg−1/d) was orally administered to rats for 15 d, the in vivo antithrombotic activity on rats
was in a concentration-dependant manner showing a significant linear relationship for ASFEtOH (P < 0.05). Meanwhile, ASFEtOH sig-
nificantly (P < 0.05) inhibited ADP-induced platelet aggregation in vivo on rats. The inhibitions of (16.3 ± 4.7)%, (28.6 ± 4.1)%, (47.9
± 4.0)% and (61.3 ± 6.6)% were reached for ASFEtOH at doses of 125, 250, 500 and 1 000 mg⋅kg−1 body weight (bw) respectively,
compared to an inhibition of (55.4 ± 7.5)% for the positive control aspirin (27 mg⋅kg−1/d). The contents of the main triterpenoid sapon-
ins 22α-hydroxychiisanoside, momordin Іb and chiisanogenin were (3 755 ± 51.3), (2 461 ± 73.0) and (1 114 ± 75.4) μg⋅g−1dry fruit
respectively. The amount of total triterpenoid saponins in ASFEtOH was (30.6 ± 0.6) mg of ginsenoside Re equivalents (GRE)/g.
CONCLUSION: These experimental results suggest that ASFEtOH has both in vivo antithrombotic and antiplatelet activities.
[KEY WORDS] Acanthopanax sessiliflorus fruits; In vivo antithrombotic activity; Antiplatelet activity; Shunt thrombosis model;
Triterpenoid; UPLC/MS/MS
[CLC Number] R965; R917 [Document code] A [Article ID] 1672-3651(2011)02-0141-05

1 Introduction
Uncontrolled platelets aggregation is critical in arterial
thrombosis which may cause life-threatening disorders such
as heart attacks, unstable angina and reocculusion after an-
gioplasty [1]. Therefore, the regulation of platelet function can
be a promising approach for the prevention or treatment of
thrombosis. Although it is well established that aspirin pro-
vides an effective secondary prevention of ischemic cardio-
vascular disorders, this drug has side effects including gas-
trointestinal symptoms and hemorrhage [2]. Thus, anti-
thrombotic agent development from medicinal plants with

[Received on] 25-Sep.-2010
[Research Funding] This project was supported by 2009 Key Labo-
ratory of Colleges and Universities Plan Scientific Research Projects
(No. 2009S101).
[*Corresponding author] LI Fa-Mei: Prof., Tel: 86-24-23986289,
Fax: 86-24-23986289, E-mail: lifamei@syphu.edu.cn
These authors have no any conflict of interest to declare.
little side effect has attracted much interest.
Acanthopanax sessiliflorus (Rupr. et Maxim) Seem. is an
indigenous plant belonging to the family Araliaceae present
mainly in China, Korea and Japan. Acanthopanax sessili-
florus has been reported to possess various pharmacological
actions such as antitumor and immunostimulating activities [3],
anti-inflammatory analgesic activity [4]. In the east of Liao
ning province of China, the fruits of Acanthopanax sessili-
florus have been developed into a series of products for food
therapy, since they are proven to be non-toxic with significant
therapeutic effects on the cardiovascular and cerebrovascular
diseases.
In our previous study [5-7], the chemical composition of
ethanol extract of Acanthopanax sessiliflorus fruits (ASFEtOH)
was investigated. It was demonstrated that three triterpenoid
saponins firstly isolated from ASFEtOH markedly inhibited
adenosine diphosphate (ADP)-induced rat platelet aggrega-
tory activities in vitro [7]. It was also reported that chiisano-
genin, oleanolic acid and acanthoside D, two triterpenoid
SONG Yang, et al. /Chinese Journal of Natural Medicines 2011, 9(2): 141−145
142 Chin J Nat Med Mar. 2011 Vol. 9 No. 2 2011 年 3 月 第 9 卷 第 2 期

saponins and a lignan isolated from Acanthopanax sessili-
florus have significant inhibitory effect on rat platelet aggre-
gation in vitro [8-9]. However, to the best of our knowledge the
antiplatelet activity of Acanthopanax sessiliflorus is mainly
investigated in the in vitro studies of individual components.
In this paper, we investigated the in vivo antithrombotic and
antiplatelet activities of ASFEtOH on rats for the first time and
determined the total triterpenoid saponin content and ten
triterpenoid saponin contents.
2 Materials and Methods
2.1 Plant source
Acanthopanax sessiliflorus fruits were collected from
Wujia Agriculture Sci-Tech. Co., Ltd. of Dandong, Liaoning
Province, China in October, 2006 and were identified by
Professor Lu Jin-Cai of School of Traditional Chinese Mate-
ria Medica, Shenyang Pharmaceutical University, China. A
voucher specimen (WGWJ 061001) was deposited in School
of Pharmacy, Shenyang Pharmaceutical University, China.
2.2 Chemicals and drugs
ADP (lot: P14216B) was purchased from Biopool Tri-
nity Biotech Plc. Bray, Co., (Wicklow, Ireland). Aspirin (lot:
88244, purity ≥ 99.0%) was supplied by Jilin Pharmaceuti-
cal Factory (Jilin, China). Sodium pentobarbital (lot: P3761)
was obtained from Sigma (St Louis, USA). Physiological
saline was purchased from Shenyang Zhiying Pharmaceutical
Factory (Liaoning, China). Sodium citrate (lot: 2000112) and
ethanol (analytical grade) were obtained from Beijing
Chemical Works (Beijing, China). Methanol and acetonitrile
(HPLC grade) were purchased from Tetia (Fairfield, OH,
USA). Ammonium acetate (HPLC grade) was obtained from
Dikma (NY, USA). Water was purified by redistillation and
filtered through 0.22 μm membrane filter before use.
ADP was diluted with physiological saline to the con-
centration of 1.2 mol·mL-1 and stored at −20 °C. Aspirin was
diluted in physiological saline to the concentration of 2.2
mg·mL-1. Sodium pentobarbital saline solution (62.5 mg·mL-1)
was freshly prepared.
Reference standards of ten triterpenoid saponins were as
follows: (1R, 11α, 22α) 1, 4-epoxy-11, 22-hydroxy-3, 4-se-
colupane-20 (30)-ene-3, 28-dioic acid (1), 22α-hydroxy-
chiisanoside (2), 22α-hydroxychiisanogenin (3), chiisanoside
(4), (1R, 11α)1, 4-epoxy-11-hydroxy-3, 4-secolupane-20
(30)-ene-3, 28-dioic acid (5), momordin Іb (6), chiisanogenin
(7), 3-O-[(α-L-arabinopyranosyl) (1→2)]-[β-D-glucurono-
pyranosyl-6-O-methyl ester]-olean-12-ene-28-olic aci (8),
oleanolic acid 3-O-6′-O-methyl-β-D-glucuronopyranoside (9),
and oleanolic acid (10). All of them were self-made [5-7].
Their purity determined using a validated UPLC-MS/MS
method was more than 98.0%.
2.3 Preparation of plant extract
Dried and powdered fruits (300 g) of Acanthopanax ses-
siliflorus were extracted with 70% ethanol of 2.5 L for 1 h a
cycle for three times. The extracts were filtered, combined
and concentrated under reduced pressure at 40 °C to obtain
ASFEtOH. The yield of ASFEtOH (145 g) was 48.3%. For ad-
ministration, the dried extract was dissolved in physiological
saline immediately before use to the designed concentration.
2.4 Determination of total triterpenoid saponin content
Total triterpenoid saponin content was determined using
the colorimetric method with vanillin–acetic acid system
which has been reported to be a simple, quick and accurate
method [10-11]. The concentration of total triterpenoid saponins
in extracts was measured by UV spectrophotometer (Shang-
hai Spectrum Instruments Co., Ltd.), according to a colori-
metric oxidation/ reduction reaction. Briefly, 1 mL of extract
solution (5 mg·mL-1) was added into a 10 mL test tube. After
the solvent was heated to evaporation in a water-bath, 0.2 mL
new mixed 5% (W/V) vanillin-acetic acid solution and 0.8
mL perchloric acid were added, mixed and incubated at 60 °C.
After the solvent was heated to evaporation in a water-bath,
0.2 mL new mixed 5% (W/V) vanillin–acetic acid solution
and 0.8 mL perchloric acid were added, mixed and incubated
at 70 °C for 15 min. The tubes were taken out and cooled in
running water for 2 min. Then ethyl acetate was added in
order to make the total volume being 5 mL. After being
cooled to room temperature, with a blank solution as refer-
ence, the absorbance was measured at 563 nm. The results
were expressed as ginsenoside Re equivalents (GRE) in mil-
ligrams per gram of extracts. Each test was repeated three
times, and the results were averaged.
2.5 Determination of ten triterpenoids of Acanthopanax
sessiliflorus
2.5.1 Liquid chromatography
The chromatography was performed on an ACQUITY
Ultra Performance LCTM system (Waters Corp., Milford, MA,
USA). The separation was achieved on an ACQUITY
UPLCTM BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm)
at ambient temperature. The gradient mobile phase was 5
mmol·L-1 ammonium acetate in water (solvent A) and ace-
tonitrile (solvent B). The following gradient was run at 0.25
mL·min-1 over 20 min. The gradient program was: 0-2 min
85% A isocratic, 2-18 min 85%-20% A, 18-19 min 20%-85%
A, 19-20 min 85% A isocratic.
2.5.2 Mass spectrometry
The mass spectrometric detection was carried out on a
Micromass® Quattro microTM API triple-quadrupole tandem
mass spectrometer (Waters Corp., Milford, MA, USA) with
an electrospray ionization (ESI) interface set in negative
ionization mode. The optimal MS parameters were as follows:
capillary voltage 2.9 kV, source temperature 120 °C and
desolvation temperature 350 °C. Nitrogen was used as the
desolvation and cone gas with a flow rate of 400 L·h-1 and 50
L·h-1 respectively. Argon was used as the collision gas. The
data collected in centroid mode were acquired and processed
using MassLynxTM NT 4.1 software with QuanLynxTM pro-
gram (Waters Corp., Milford, MA, USA). The quantifica-
tion was performed using selective reaction monitoring
SONG Yang, et al. /Chinese Journal of Natural Medicines 2011, 9(2): 141−145
2011 年 3 月 第 9 卷 第 2 期 Chin J Nat Med Mar. 2011 Vol. 9 No. 2 143

(SRM). The transition, cone voltage and collision energy for
the determination of ten triterpenoid saponins in the plant
material are shown in Table 1.
The contents of the ten triterpenoid saponins in the plant
material were determined using a validated UPLC-MS/MS
method. The linear relationships for the responses of all ten
standards were proved. The method was shown to be valid
with intra- and inter-day precision (relative standard devia-
tion, RSD.) values within 2.8% and 2.4%, and acceptable
recovery of 95.1%-104.7% for the ten compounds. The ma-
trix effects and stability were also tested and proven to be
acceptable. These validation results demonstrated the suit-
ability of the method for the precise and accurate determina-
tion of Acanthopanax sessiliflorus fruit.
Table 1 MRM transition in negative mode, cone voltage and collision energy for the determination of ten triterpenes in Acan-
thopanax sessiliflorus fruits
No. Compound Transition Cone voltage/ V Collision energy/eV
1 (1R, 11α, 22α) 1, 4-epoxy-11, 22-hydroxy-3, 4- secolupane-20 (30)-ene-3, 28-dioic acid 517→56 50 40
2 22α-hydroxychiisanoside 969→499 50 30
3 22-α-hydroxychiisanogenin 499→349 50 50
4 chiisanoside 953→483 50 40
5 (1R, 11α)1, 4-epoxy-11-hydroxy-3, 4-secolupane- 20 (30)-ene-3, 28-dioic acid 501→457 50 40
6 momordin Іb 631→455 50 50
7 chiisanogenin 483→58 50 50
8 3-O-[(α-L-arabinopyranosyl) (1→2)]-[β-D-glucuronopyranosyl- 6-O-methyl ester]-olean-12-ene-28-olic acid 777→455 60 10
9 oleanolic acid 3-O-6′-O-methyl-β-D-glucuronopyranoside 645→455 60 10
10 oleanolic acid 455→375 60 10

2.6 Experimental animals
Male Wistar rats weighing 240-280 g were obtained
from the animal center of Shenyang Pharmaceutical Univer-
sity, which were used after overnight fasting.
2.7 Animal treatments
For the thrombosis and the platelet aggregation tests, 36
Wistar rats were divided randomly into six groups: control,
aspirin and four ASFEtOH groups in different doses (125, 250,
500 and 1 000 mg·kg-1 bw). It is reported that LD50 of mice
intraperitoneally injected Acanthopanax sessiliflorus ethanol
extract solution is 13 g·kg-1 [12]. The animals were treated
orally with the corresponding agents for 15 consecutive days.
The thrombosis model and the platelet aggregation were
tested 1h after the last administration.
2.8 Thrombosis model
The rat arteriovenous shunt thrombosis model [13-14] was
modified for the present study and the shunt tube was pre-
pared from the polyethylene tubing of scalp vein set. After
anaesthesia with sodium pentobarbital (50 mg·kg-1, i.p.), an 8
cm polyethylene tube (internal diameter 1.5 mm) was in-
serted between the left jugular vein and the right carotid ar-
tery. The saline-filled shunt was assembled by connecting
two cannulae with a slightly curved 6 cm-long tygon tubing
(internal diameter 2 mm) containing a 5 cm-long cotton
thread (diameter 0.25 mm). The extracorporeal circulation
was maintained for 15 min, during which a thrombus adhered
to the cotton thread. The shunt was then removed and the
thread with its associated thrombus was withdrawn and im-
mediately weighed. The thrombus wet weight was deter-
mined by subtracting from the value obtained the weight of
the cotton thread determined previously.
2.9 Preparation of platelet-rich and platelet-poor plasma
Blood was obtained from the carotid artery after surgery
through the cannulae. The freely flowing blood was dis-
pensed into a centrifuge tube containing 0.3 mL sodium cit-
rate for each 2.7 mL of blood. The citrated blood was mixed
well by gentle inversion. Blood samples were centrifuged at 1
200 r·min-1 for 10 min at room temperature. The supernatant
platelet-rich plasma (PRP) was isolated. The residue was
centrifuged at 3 000 r min-1 for 10 min at room temperature
to obtain platelet-poor plasma (PPP).
2.10 In vivo platelet aggregation assay
The platelet aggregatory rates were determined on an
LG-KOALA platelet aggregator (Beijing Steellex Tech Instru
Co., Ltd. China) following Born’s turbidimetric method [15].
Aggregation was recorded as the percent change in light
transmission: the baseline was set using PRP and maximal
transmission using PPP. PRP of 200 μL was added into the
microbasin. After pre-incubation at 37.8 °C for 2 min in the
aggregometer, 1.5 μL of ADP was added to the cell and ag-
gregation curves were recorded for 5 min. Tests were per-
formed within 3 h to avoid platelet inactivation. The effects
of the extract and aspirin were expressed as percent inhibition
compared with control samples. Maximum aggregation was
recorded for the control (CA) and the different tests (TA).
The inhibition of aggregation (IA) was calculated as: IA =
100 (CA-TA) / CA.
2.11 Statistical analysis
ANOVA was used to evaluate the differences between
groups administered with various concentrations of ASFEtOH
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144 Chin J Nat Med Mar. 2011 Vol. 9 No. 2 2011 年 3 月 第 9 卷 第 2 期

and control and aspirin groups in the platelet aggregation and
thrombus weight. A two-tailed P value below 0.05 was con-
sidered as a significant difference. All the numerical values
were expressed as x ± s.
3 Results and Discussion
3.1 Total triterpenoid saponin content in ASFEtOH
The total triterpenoid saponin content was determined
according to the colorimetric method with vanillin-acetic acid
system and expressed as GRE. The amount of total triterpe-
noid saponins in ASFEtOH was (30.6 ± 0.6) mg of GRE/g.
Many studies have revealed that the triterpenoid saponin
content in plants are related to their antithrombotic and anti-
platelet activities [7-9], which demonstrated that the anti-
thrombotic and antiplatelet activities of ASFEtOH were proba-
bly attributed to its triterpenoid saponin compounds.
3.2 Quantification of ten triterpenoid saponins in the fruit
extract
The contents of compound 1-10 in Acanthopanax sessili-
florus fruit shown in Table 1 are (38.7 ± 0.501), (3 755 ±
51.3), (355 ± 12.3), (443 ± 17.9), (61.2 ± 5.04), (2 461 ±
73.0), (1 114 ± 75.4), (1.50 ± 0.023 6), (1.62 ± 0.084 2) and
(12. 9 ± 0.729) μg·g-1 dry fruit respectively. The result shows
that 22α-hydroxychiisanoside, momordin Іb and chiisano-
genin are the major components accounted for 89.0% among
the ten triterpenoid saponins. It was reported that chiisano-
genin and oleanolic acid had significant inhibitory effect on
rat platelet aggregation in vitro [7-8]. Apart from chiisanogenin,
the other two main triterpenoid saponins 22α-hydroxy-
chiisanoside and momordin Іb are deratives of chiisanogenin
and oleanolic acid. Meanwhile our previous study has proved
that compounds 1, 5 and 8 shown in Table 1 markedly inhib-
ited ADP-induced rat platelet aggregatory activities in vitro [7].
The aforesaid results demonstrated that the antiplatelet ac-
tivity of triterpenoid saponins were probably due to the main
triterpenoid saponins 22α-hydroxychiisanoside, momordin Іb
and chiisanogenin as well as some smaller amounts of the
related triterpenoid saponins. More scientific work needs to
be done regarding the three triterpenoid saponins in order to
further verify their antithrombosis and antiplatelet effects in
in vivo conditions.
3.3 Effects on the thrombus formation
Compared with the control group, a 15-day treatment
with ASFEtOH decreased thrombus weight in rat arteriovenous
shunt thrombosis model. As shown in Fig. 1, the thrombus
weight of all ASFEtOH treated groups and aspirin group were
significantly different from that of the control group (P <
0.05). A significant linear correlation between the dose of
ASFEtOH and thrombus weight was observed (y = −0.0483 x +
91.446, r = 0.958 4) indicating a dose-dependent effect.
When 1 000 mg·kg-1 bw of ASFEtOH was administered orally
to rats for 15 d, the thrombus weight was decreased by the ex-
tract more than by 27 mg·kg-1 bw aspirin with respect to control.
3.4 Effects on the platelet aggregation
The ADP-induced platelet aggregatory inhibitions (%)

Fig. 1 Thrombus weight (mg) in arteriovenous shunt
thrombosis rat model 1 h after last administration of 15-day
treatment with 0.9 % saline (control), ASFEtOH (125, 250, 500,
1 000 mg·kg-1 body weight) or aspirin (27 mg·kg-1 body
weight) (n = 6), * P < 0.05, ** P < 0.01 vs control
were determined after orally administered with 125, 250, 500
and 1 000 mg·kg-1 bw ASFEtOH or 27 mg·kg-1 bw aspirin
alone as (16.3 ± 4.7)%, (28.6 ± 4.1)%, (47.9 ± 4.0)%, (61.3 ±
6.6)% and (55.4 ± 7.5)%, respectively. ASFEtOH was also
found to inhibit the ADP-induced platelet aggregation in a
concentration dependent manner after a 15-day administra-
tion to rats, as shown in Fig. 2. The ADP-induced platelet
aggregatory inhibitions of all ASFEtOH treated groups and
aspirin group were significantly different from that of the
control group (P < 0.05). There was a significant linear cor-
relation between the inhibition to platelet aggregation and the
dose of ASFEtOH (y = 0.049 4 x + 15.4, r = 0.956 7). Treat-
ment with aspirin as positive control with potent antithrom-
botic activity at the dose of 27 mg·kg-1 bw and a treatment
with a dose of 500 mg·kg-1 bw ASFEtOH produced a similar
effect (P > 0.05).

Fig. 2 The ADP-induced platelet aggregatory inhibitions (%)
after last administration of 15-day treatment with aspirin (27
mg·kg-1 body weight) or ASFEtOH (125, 250, 500, 1 000 mg·kg-1
body weight) (n = 6),* P < 0.05, ** P < 0.01 vs control
Above all, ASFEtOH of different doses ranging from 125
mg·kg-1 bw to 1 000 mg·kg-1 bw possessed both antithrom-
bosis and antiplatelet effects in a dose-dependent manner in
vivo on rats. The total triterpenoid saponin content of differ-
ent doses of ASFEtOH (125, 250, 500 and 1000 mg·kg-1 bw)
SONG Yang, et al. /Chinese Journal of Natural Medicines 2011, 9(2): 141−145
2011 年 3 月 第 9 卷 第 2 期 Chin J Nat Med Mar. 2011 Vol. 9 No. 2 145

were 7.97, 15.94, 31.88 and 63.75 mg·kg-1 bw respectively.
As the effect of an oral dose of 500 mg·kg-1 bw ASFEtOH was
equivalent to that of 27 mg·kg-1 bw aspirin as positive control
in rats, a dose of 31.88 mg·kg-1 bw total triterpenoid saponins
probably produced a similar effect with aspirin in a dose of
27 mg·kg-1 bw.
4 Conclusion
ASFEtOH showed significant antithrombotic and anti-
platelet activities in vivo. This novel work provides an impe-
tus for conducting clinical studies on the potentially bene-
ficial hematological effects of ASFEtOH. This phytomedicine
may also be an important source of lead compounds for the
development of novel therapeutics.
Acknowledgements
We acknowledge Dr. Zhou Xiao-Mian (Department of
Pharmacology of Shenyang Pharmaceutical University) for
the assistance in bioactivity experiment.
We are grateful to President Sun Bao-Jun (Wujia Agri-
culture Sci-Tech Co., Ltd. of Dandong) for providing the
Acanthopanax sessiliflorus fruits.
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定量无梗五加果提取物的体内抗血栓与抗血小板凝集活性
宋 洋, 杨春娟, 于 凯, 李发美*
沈阳药科大学分析化学教研室, 沈阳 110016
【摘 要】 目的：研究无梗五加果乙醇提取物的大鼠体内抗血栓与抗血小板凝集活性及其三萜皂苷类成分。方法：采用大
鼠动-静脉旁路血栓模型的测定无梗五加果醇提物的体内抗血栓活性; 采用血小板凝集仪测定其体内抗 ADP 诱导的血小板凝集
活性; 采用 UPLC-MS/MS 法同时对无梗五加根提取物中 10 种三萜皂苷类成分进行含量测定并采用比色法对其总三萜皂苷成分
的含量进行了测定。结果：连续灌胃给予大鼠无梗五加醇提物(125, 250, 500 及 1 000 mg⋅kg−1/d)15 天后, 其体内抗血栓活性显示
出剂量依赖关系(P < 0.05)。同时, 提取物也显著抑制了大鼠体内 ADP 诱导的血小板凝集活性(P < 0.05)。不同剂量提取物(125, 250,
500 及 1 000 mg/kg⋅d−1)下的抑制率分别为(16.3 ± 4.7)%, (28.6 ± 4.1)%, (47.9 ± 4.0)% 与 (61.3 ± 6.6)%, 阳性对照阿司匹林组(27
mg⋅kg−1/d)的抑制率为(55.4 ± 7.5)%。提取物中 3 个主要三萜皂苷成分 22α-hydroxychiisanoside, momordin Іb 以及 chiisanogenin
的含量分别为(3 755 ± 51.3), (2 461 ± 73.0)和(1 114 ± 75.4) μg·g-1。以人参皂苷 Re 计, 无梗五加果中总皂苷含量为(30.6 ± 0.6)
mg·g-1。结论：无梗五加果醇提物具有大鼠体内抗血栓与抗血小板凝集活性。
【关键词】 无梗五加果; 体内抗血栓活性; 抗血小板凝集活性; 旁路血栓模型; 三萜; UPLC-MS/MS

【基金项目】 辽宁省教育厅科学技术研究重点实验室项目(No. 2009S101)