Molecular Cloning and Construction of agp Gene Deletion-mutant in Cyanobacterium Synechocystis sp. PCC 6803 Rapid Development of Glu-1 Locus Near-isogenic Introgression Lines Using HMW-GS Deletion Mutant Studies on Crystalline Growth of MoFe Protein from a nifZ Deleted Strain of Azotobacter vinelandii Cloning the Deleted DNA Fragment from a Deletion Mutant of Planetree by Genomic Subtraction