Abstract:Gordonia acuminata is a dominant tree species of subtropical evergreen broad-leaved forest on Mt. Jinyun,Sichuan. This study analyzed pollen free proline content and seed yield of G. acuminata in three different communities located in Mt. Jinyun. Results showed that pollen free proline content and seed yield per fruit of G. acuminata were not significantly different among G. acuminata plants of different ages growing in the same community, but were significantly different among three communities. Pollen free proline content and seed yield per fruit of G. acuminata plants showed highest in evergreen broad-leaved forest, lowest in Pinus massoniana-G. acuminata mixed forest, and intermediate in G. acuminata forest. Pollen free proline content of G. acuminata was closely positively correlated to seed yield per fruit of G. acuminata and this suggests that pollen free proline content can be used as an index for predicting and comparing seed yield of G. acuminata plants in different eco-environments of same region.