该文报道了乙烯产生抑制剂氯化钴(CoCl2)及氨基乙基乙烯基甘氨酸（Aminoethoxyvinylglycine, AVG）对高温胁迫下蚕豆(Vicia faba)幼苗叶片保护作用的现象及机制。研究结果表明，用CoCl2和AVG溶液处理，在高温胁迫前及胁迫12 h时皆显著抑制乙烯产生。20 μmol·L-1 CoCl2浇灌处理在高温胁迫前可以显著提高叶片抗氧化酶SOD、CAT、APX的酶活力，而20 μmol·L-1 AVG处理对这些抗氧化酶活力无显著影响。20 μmol·L-1 CoCl2 与20 μmol·L-1 AVG 溶液浇灌处理在高温胁迫前对腐胺(Put)、亚精胺(Spd)、精胺(Spm)、叶绿素含量、活性氧（ROS）产生速率和脂质过氧化程度无显著影响。但经45 ℃高温胁迫12 h后，20 μmol·L-1 CoCl2 与20 μmol·L-1 AVG溶液处理显著提高了上述3种多胺的含量，减缓了抗氧化酶活性的下降，对活性氧水平及脂质过氧化水平的增加也起到了有效的抑制作用，这些改变可能对叶绿素降解的减少及膜稳定指数下降的减轻发挥着重要的作用。可见，通过抑制乙烯产生，相对提高多胺含量和抗氧化酶活性，以及抑制活性氧水平的提高和抑制细胞膜稳定指数的下降，CoCl2和AVG对高温胁迫下的蚕豆幼苗叶片产生了一定的保护作用。
Here, we studied the effects of ethylene production inhibitors (cobalt chloride (CoCl2) and aminoethoxyvinylglycine (AVG)) on the leaves of Vicia faba seedlings under heat stress. Ethylene production in the leaves was inhibited significa ntly before heat stress and after 12 h heat stress when the seedlings were irrigated with 20 μmol·L-1 CoCl2 or 20 μmol·L-1 AVG solution for 7 days. Treatment with 20 μmol·L-1 CoCl2 increased significantly the activities of SOD, CAT and APX before heat stress, while the treatment with 20 μmol·L-1 AVG had no significant influences. Treatments with 20 μmol·L-1 CoCl2 and 20 μmol·L-1 AVG did not influence the contents of putrescine (Put), spermidine (Spd), spermine (Spm), chlorophyll, and the production rate of reactive oxygen species (ROS). However, after 12 h heat stress of 45 ℃, treatments significantly promoted the above values. These results showed that when plants suffered 12 h heat stress of 45 ℃, treatment with 20 μmol·L-1 CoCl2 and 20 μmol·L-1 AVG inhibited ethylene production, increased the content of these amines and enhanced the activities of the anti_oxidative enzymes, restrained the enhancement in the content of ROS, and alleviated the reduction in membrane stability index, by which the leaves of Vicia faba seedlings were protected.