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EFFECT OF SALT STRESS ON GERMINATION OF BROMUS STAMINEUS AND BROMUS INERMIS CV. XILINGUOLE

盐胁迫对两种无芒雀麦种子萌发的影响


在实验室中对两种无芒雀麦本地种(Bromus inermis cv. Xilinguole)和引进种 (Bromus stamineus)种子在浓度为0.5%、1.0%、1.5%的Na2SO4、NaCl和MgCl2 3种单盐胁迫下进行发芽实验,结果表明:与对照相比,在以上3种盐胁迫下,随着胁迫浓度的增加,两种无芒雀麦种子的发芽率、发芽指数和发芽值均有不同程度的降低,相对盐害率升高,种子开始发芽的时间推迟且其发芽过程延长;在3种盐中,NaCl的胁迫对两种植物种子萌发的抑制作用最大,其中浓度为1.0% NaCl的胁迫对引进种和本地种无芒雀麦发芽率的抑制分别达到了68.67%和14%,在浓度为1.5%的NaCl胁迫下两种供试植物的发芽率均为零;通过建立不同盐分处理与两种无芒雀麦种子发芽率之间的回归方程得出,引进无芒雀麦种子芽期的耐盐性较本地无芒雀麦强。

Aims Use of saline land has become a major agricultural issue around the world. China has 27 millions kilometer saline land, and the area is increasing. Our objective was to compare the germination ability of the saline-tolerant Bromus stamineus from the USA and B. inermis cv. Xilinguole under saline conditions.
Methods We applied Na2SO4, NaCl and MgCl2 at concentrations of 0.5%, 1.0% and 1.5% for salt stress treatments and recorded germination percentage, germination energy, germination index, germination value etc. We put seeds of the two plants in culture dishes covered with a filter paper and a glass bell jar with a small hole. Each culture dish was put on a beaker containing different salt solution, with gauze connecting the solution and the filter paper. The gauze extended outside the bell jar to evaporate and avoid salt accumulation on the filter paper, keeping the germination environment constant. 
Important findings Germination percentage, germination index and germination value for both species declined with increased salinity, with values reduced more for B. inermis cv. Xilinguole than for B. stamineus. Times for seeds to germinate and complete their germination were prolonged under high salinity stress. NaCl had greater effects than Na2SO4 and MgCl2 on both species. Overall, B. stamineus exhibited greater germination ability with salt stress than B. inermis cv. Xilinguole.