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卞 坡 !! 苏明杰 ! 秦广雍 ! 余增亮 !! 霍裕平
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!!!!23+ ’(+ /01 的 234离子束在 !5-!!(!6% 789:;<=的注入剂量下介导外源甘蓝全 >?2 导入模式植物拟南芥菜!
在 @, 株转化当代植株中! 有 A 株表型产生变异 以其中的一株BCD-.作为研究对象! 用 E( 条 !( 碱基随机引物对
该株和其子代变异株基因组作随机扩增的多态性 >?2 分析! 引物 F!6A+ 在 CD- 和其变异子代 CD-D 中扩增出了相
同分子量的变异新条带 CD-F!6ADA( CD-F!6ADA( 的碱基序列和拟南芥菜基因组序列进行同源比对! 结果表明该
片段不属于拟南芥菜基因组! F8GHI039 杂交实验证明该片段来自供体甘蓝基因组 但是! 根据 CD-F!6ADA( 序列
设计的引物不能从甘蓝基因组中扩增出预期长度的 >?2 片段! 结合离子束介导外源全 >?2 转化的特点和过程!
探讨了其中可能的机制
456!低能 23J离子介导外源全 >?2 转化)% 拟南芥菜)% 随机扩增的多态性 >?2)% 同源比对
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