全 文 ：Research on Se Content of Different Tartary
Buckwheat Genotypes
SHI Zheng，HAN Cheng-hua，HUANG Kai-feng*
Institute of Plant Genetics and Breeding，School of Life Science，Guizhou Normal University，Guiyang 550001
Abstract ［Objective］The aim was to screening the tartary buckwheat resources with higher Se content． ［Method］35 accessions of tartary
buckwheat resources were used as experimental materials，and the Se content of seeds were determined． ［Result］The Se content of 35 kinds
of tartary buckwheat will change in a range of 0． 009 9 to 0． 120 8 mg/g with an average of 0． 040 6 mg/g． There was difference among tartary
buckwheat from different origins． The seeds from Gansu had the highest content of Se，while the seeds from Nayong in Guizhou and Shaanxi
had a lowest content of Se． ［Conclusion］The study had important significance for further study on the genetic and variation law of Se content
between different tartary buckwheat resources．
Key words Tartary buckwheat; Se content; Genetic variation
Received: January 21，2011 Accepted: February 28，2011
Supported by Science and Technology Foundation of Guizhou Prov-
ince［Qiankehe-J ( 2009 ) No． 2108 ) ］; Guizhou Normal University
Fund for Doctor ( 2008) ．
* Corresponding author． E-mail: hkf1979@163． com
Tartary buckwheat belongs to Genus Fagopyrum of Po-
lygonaceae，also known as Fagopyrum tataricum Gaertn．［1］．
As one of the minor crops in China，it is a good catch crop
and important nectar crop． It has a short growing period［2］．
Tartary buckwheat has a high nutritional and medicinal value．
It has high protein content and is rich in all kinds of essential
amino acids． Moreover，the composition of its protein is rea-
sonable with appropriate ratio，which is close to the standard
protein． Its nutrition is higher than other cereal crops［3 －5］． It
has high nutritional value as its seed protein，fat，vitamins and
trace elements contents are generally higher than rice，wheat
and other major food crops． Moreover，tartary buckwheat
seeds also contain the bio-flavonoids which are not existed in
other cereal crops［6］． As the tartary buckwheat has nutrition，
health and medical care prospects，it has been known as the
21st centurys most popular green food［7 －8］． This is the main
reason for the rapid demand of tartary buckwheat from the do-
mestic and international market． Therefore，in recent years，
the development and application of tartary buckwheat have
been paid more and more attention． Many provinces，espe-
cially the southwest China，have considered it as a unique
small grain for planting and spreading．
Selenium is essential trace element for human． Medical
research confirmed that a lot of human diseases were related
to long-term inadequate intake of selenium［9］． Keshan dis-
ease and Kashin-Beck disease in China have been confirmed
as selenium deficiency disease． In addition，Se is related to
cancer，cardiovascular and cerebrovascular diseases，
AIDS，diabetes and other 40 kinds of diseases［6］． It is thought
that appropriate selenium can enhance human resistance to
these diseases． Therefore，the breeding of tartary buckwheat
varieties ( lines ) with high Se content showed great pros-
pects． However，there are a lot of tartary buckwheat re-
sources． Therefore，35 accessions of tartary buckwheat re-
sources from different origins under the same cultivation con-
ditions were used as materials，and the Se content was detec-
ted to explore the genetic variation law of protein content a-
mong different varieties of tartary buckwheat resources so as
to provide theoretical basis for the breeding of new varieties of
tartary buckwheat with higher Se content as well as its devel-
opment and utilization．
Materials and Methods
Materials
35 accessions of tartary buckwheat resources ( Table 1)
were provided by Institute of Plant Genetics and Breeding，
School of Life Science，Guizhou Normal University． All mate-
rials are planted in the Boyang experimental base of Institute
of Plant Genetics and Breeding，School of Life Science，
Guizhou Normal University in Longdongbao of Guizhou on Au-
gust 20，2010． Each material was planted in a plot with the
plot area of 2 × 5 m，spacing of 40 cm and plant spacing of
10 cm，and the conventional field management was carried
out． Seeds were harvested on November 30，2010，after
dried，they were stored at －20 ℃．
Table 1 35 copies of experimental tartary buckwheat resources
No． Cultivar Origin Source Symbol
1 Daku Weining Guizhou Institute of Plant Genetics and Breeding T282
2 Pingbi2 Weining Guizhou Institute of Plant Genetics and Breeding T284
3 Heiku Hezhang Guizhou Institute of Plant Genetics and Breeding T292
4 DanxuanF01 Weining Guizhou Institute of Plant Genetics and Breeding T301
5 Ludian Yunnan Institute of Plant Genetics and Breeding T305
Methods
The tartary resources stored at － 20 ℃ freezer were
taken out into 105 ℃ oven for 15 min of green removing，and
then they were dried to constant weight at 60 ℃． After shell-
ing，they were milled by milling and saved in dryer． Before
Agronomy
Agricultural Science ＆ Technology，2011，12( 1) : 102 －104，156
Copyright 2011，Information Institute of HAAS． All rights reserved．
DOI:10.16175/j.cnki.1009-4229.2011.01.024
To be continued ( Table 1)
No． Cultivar Origin Source Symbol
6 DanxuanF02 Weining Guizhou Institute of Plant Genetics and Breeding T309
7 Yimengku Weining Guizhou Institute of Plant Genetics and Breeding T324
8 Baizhengku Weining Guizhou Institute of Plant Genetics and Breeding T330
9 Ku37 Weining Guizhou Institute of Plant Genetics and Breeding T340
10 Chuanku1 Sichuan Institute of Plant Genetics and Breeding T345
11 Saobai Weining Guizhou Institute of Plant Genetics and Breeding T349
12 K013 Weining Guizhou Institute of Plant Genetics and Breeding T352
13 Ciku Weining Guizhou Institute of Plant Genetics and Breeding T364
14 86-36 Weining Guizhou Institute of Plant Genetics and Breeding T365
15 Pujiangku Liupanshui Guizhou Institute of Plant Genetics and Breeding T367
16 19-22 Weining Guizhou Institute of Plant Genetics and Breeding T373
17 Wugeheiku Weining Guizhou Institute of Plant Genetics and Breeding T374
18 KP005 Shaanxi Institute of Plant Genetics and Breeding T384
19 Qianwei 1 Weining Guizhou Institute of Plant Genetics and Breeding T388
20 Heiku 6 Weining Guizhou Institute of Plant Genetics and Breeding T395
21 Xibaiku Weining Guizhou Institute of Plant Genetics and Breeding T398
22 Longku Weining Guizhou Institute of Plant Genetics and Breeding T412
23 Shanku Hezhang Guizhou Institute of Plant Genetics and Breeding T415
24 Caobaku Hezhang Guizhou Institute of Plant Genetics and Breeding T416
25 1Ku1 Hezhang Guizhou Institute of Plant Genetics and Breeding T425
26 Zhuangku Hezhang Guizhou Institute of Plant Genetics and Breeding T429
27 Dachongku2 Weining Guizhou Institute of Plant Genetics and Breeding T441
28 Xueshanku Weining Guizhou Institute of Plant Genetics and Breeding T444
29 Yilaku Weining Guizhou Institute of Plant Genetics and Breeding T446
30 Weiku02-286 Weining Guizhou Institute of Plant Genetics and Breeding T452
31 Xinong6-14 Shaanxi Institute of Plant Genetics and Breeding T453
32 Ping01-043 Gansu Institute of Plant Genetics and Breeding T460
33 Yunku67 Yunnan Institute of Plant Genetics and Breeding T463
34 Yuku2 Shaanxi Institute of Plant Genetics and Breeding T468
35 Nayongku Nayong Guizhou Institute of Plant Genetics and Breeding T470
determination，they were dried to constant weight again in ov-
en at 60 ℃． The detection of se content was referred to GBIT
5009．93 －2003［10］．
Data processing
Excel 2003 software was used for data processing，and
SPSS 17．0 was used for significant difference analysis on data．
Results and Analysis
Comparison of Se content in different tartary buckwheat
materials
Table 2 showed that the Se content in 35 accessions of
tartary buckwheat materials ranges from 0． 009 9 to 0． 120 8
mg/kg with an average of 0． 040 6 mg/kg，in which the T460
from Gansu had the highest Se content; while T412 from Wein-
ing，Guizhou showed the lowest Se content． According to Se
content in 35 accessions of tartary buckwheat materials ( Table
3) ，these materials could be divided into three types: low Se
content type，the Se content was below 0． 019 2 mg/kg，inclu-
ding two accessions ( accounting for 5． 71% of the total of ex-
perimental materials) ; middle Se content type，the Se content
was in the range of 0．019 2 to 0．074 7 mg/kg，including 31 ac-
cessions ( accounting for 88． 58% of the total of experimental
materials) ; high Se content type，the Se content was above
0．074 7 mg/kg，including two accessions of materials ( account-
ing for 5．71% of the total of experimental materials) ．
Table 2 Comparison of content of Se content of different tartary
buckwheat resources mg/kg
No． Se content No． Se content No． Se content
1 0． 041 2 13 0． 090 4 25 0． 064 1
2 0． 044 4 14 0． 010 2 26 0． 019 4
3 0． 061 5 15 0． 035 8 27 0． 018 6
4 0． 036 4 16 0． 017 0 28 0． 057 0
5 0． 042 4 17 0． 022 6 29 0． 052 4
6 0． 020 8 18 0． 016 4 30 0． 022 5
7 0． 058 8 19 0． 049 2 31 0． 032 6
8 0． 049 2 20 0． 067 3 32 0． 120 8
9 0． 030 9 21 0． 037 6 33 0． 071 7
10 0． 046 8 22 0． 009 9 34 0． 018 5
11 0． 062 5 23 0． 015 3 35 0． 023 7
12 0． 023 5 24 0． 028 3
Comparison of Se content in seeds of tartary buckwheat
from different origin
Table 4 suggested that there were some differences on
Se content of tartary buckwheat from different origin，in which
the Se content of tartary buckwheat from Gansu was higher to
0． 120 8 mg/kg; while the Se content of tartary buckwheat
from Nayong of Guizhou and Shaanxi was lower，which was
0． 023 7 and 0．025 5 mg/kg，respectively． There was signifi-
cant difference on Se content of tartary buckwheat seeds from
different origin． Besides，it was also found that the variation
301SHI Zheng et al． Research on Se Content of Different Tartary Buckwheat Genotypes
coefficient of Se content of tartary buckwheat seeds from Yun-
nan was the lowest，indicating that it was more stable． How-
ever，the variation coefficient of Se content of tartary buck-
wheat seeds from Hezhang of Guizhou was the highest．
Table 3 Frequency distribution of Se content
No．
Interval
mg /g
Group median
mg/kg
Frequence f
Cumulative
frequence Cf
Frequency
Cumulative
Frequency Cr
1 0． 000 7 －0． 019 2 0． 010 0 2 2 0． 057 1 0． 057 1
2 0． 019 2 －0． 037 7 0． 028 5 17 19 0． 485 7 0． 542 8
3 0． 037 7 －0． 056 2 0． 047 0 7 26 0． 200 0． 742 8
4 0． 056 2 －0． 074 7 0． 065 5 7 33 0． 200 0． 942 8
5 0． 074 7 －0． 093 2 0． 084 0 1 34 0． 028 6 0． 974 4
6 0． 093 2 －0． 111 7 0． 102 5 1 35 0． 028 6 1． 000 0
Table 4 Se content in different origins of tartary buckwheat seeds and its multiple comparison results
Origin Number of samples
Minimum of Se content
mg /kg
Maximum of Se content
mg /kg
Mean CV∥%
Weining Guizhou 21 0． 009 9 0． 090 4 0． 039 2 cd 53． 80
Hezhang 5 0． 015 3 0． 064 1 0． 037 7 d 62． 08
Liupanshui 1 － － 0． 035 8 d －
Nayong 1 － － 0． 023 7 e －
Sichuan 1 － － 0． 046 8 c －
Shaanxi 3 0． 016 4 0． 032 6 0． 025 5 e 38． 99
Gansu 1 － － 0． 120 8 a －
Yunnan 2 0． 042 4 0． 071 7 0． 057 1 b 36． 26
Different lower case letters mean significant difference at 0． 05 level．
Conclusions and Discussions
TANG Yu-xia et al．［11］ used the national standard method
GB/T 5009． 93 － 2003 for the determination of Se content in
three kinds of wheat grain and found that the range of its con-
tent was 0． 051 to 0． 220 mg/kg． JIANG Chuan et al．［12］ used
a total of 45 accessions of rice germplasm resources utilized
in Fujian Province Production as experimental materials to
study the Se content in rice，and the results showed that there
was great difference on Se content in rice of different geno-
types ( the variation amplitude was 0．009 to 0． 065 mg/kg with
an average of 0． 067 mg/kg ) ． The results in this study
showed that the variation amplitude of Se content of 35 acces-
sions of tartary buckwheat was 0． 009 9 －0．120 8 mg/kg with
an average of 0． 040 6 mg/kg，which was consistent with the
result of CHEN Qing-fu［13］ but generally lower than the result
of HUANG Xiao-yan et al．［6］． These differences might be due
to the involved different measurement methods． In these 35
accessions of tartary buckwheat，the T460 from Gansu showed
the highest Se content ( 0．120 8 m/kg) ，which had not excee-
ded the state limit of Se content 0． 3 mg/kg［11］，thus should be
further spread and applied in the development and utilization of
tartary buckwheat resources with high Se content．
In addition，the material used in this study were from the
same plot with the same climatic conditions，but the result
showed that the Se content among different varieties were sig-
nificantly different，which might be caused by genetic factors
among the buckwheat materials．
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Responsible editor: LI Ting-ting Responsible proofreader: WU Xiao-yan
( 下转第 156 页)
401 Agricultural Science ＆ Technology Vol． 12，No．1，2011
Conclusions
Based on Supermap platform and Google Earth API tech-
nology，the basic information database and WEBGIS platform
of traditional village were constructed，and the planning con-
trol key data system of traditional village was formed，while
comprehensive management platform based on GIS was es-
tablished so as to make up for the weak link of protecting tra-
ditional village in China． By using computer network，the basic
information data of traditional village were saved and managed
scientifically according to related national standard and specifi-
cation． According to the feature，information and digital devel-
opment of traditional village in different regions，the basic in-
formation database of traditional village was established
through investigating typical traditional settlement，and the for-
mation，development and basic law of various traditional villa-
ges were studied in our paper． Realizing rapid acquisition，
building database，query，retrieval，timely update，statistics，
spatial analysis and resources sharing，dynamic basic data
were changed into digital，operable and sharable information
resource，which could provide decision information for urban
development．
At present，our system needs further improvement in
some aspects［7］，for example，data redundancy may appear
in the inputting progress of national data and result into low
query efficiency，so the index optimization of spatial data
needs further improvement; when the system is applied in ad-
ministrative units at various levels，its access control needs
further study according to the demand of actual business;
three-dimensional virtual data can be added in system based
on VRML，and card should be installed in browser，so FLEX
technology should be used in further study．
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檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪
WU Xiao-yan
基于 GIS的中国传统村镇管理系统设计和实施
于明洋* ，张子民，史同广 ( 山东建筑大学土木工程学院，山东济南 250101)
摘要 针对目前中国在经济村落保护方面的薄弱环节，基于地理信息系统( GIS) 、Google API技术与 Supermap I Server Java 2008开发平台，对
中国传统村镇WEBGIS管理平台的构建与实现进行探讨。通过对中国传统村镇WEBGIS管理平台的构建，提高了基本信息数据库数据的采
集、更新及入库效率，进一步节省了工作成本，便于相关部门的监督和决策，实现了部分工作的动态、分级及透明管理，对乡镇村落的现代化
建设和管理工作具有重大意义。
关键词 传统村镇;WEBGIS; Supermap;决策支持
基金项目 国家“十一五”科技支撑计划课题( 2006BAJ04A03-01)。
作者简介 于明洋( 1978 － ) ，男，山东东河人，在读博士，讲师，从事地理信息系统、遥感等研究。* 通讯作者。
收稿日期 2010-12-15 修回日期
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2011-02-15
( 上接第 104页)
苦荞种子中硒含量的基因型差异研究( 摘要)
时政，韩承华，黄凯丰* ( 贵州师范大学生命科学学院植物遗传育种研究所，贵州贵阳 550001)
［目的］筛选出硒含量较高的苦荞资源。
［方法］以不同产地的 35份苦荞资源为试验材料，测定了其籽粒中的硒含量。
［结果］35份苦荞资源的硒含量变化幅度为0． 009 9 －0． 120 8 mg /g，平均值为0． 040 6 mg /g;不同产地苦荞的硒含量存在差异，以甘肃地区较
高，贵州纳雍和陕西较低。
［结论］为进一步研究硒含量在不同苦荞资源间的遗传变异规律提供了理论依据。
关键词 苦荞;硒含量; 遗传变异
基金项目 贵州省科学技术基金项目［黔科合 J字( 2009) 2108号］;贵州师范大学博士科研基金项目( 2008年)。
作者简介 时政( 1983 － ) ，男，讲师，硕士，从事营养保健研究，E-mail: drshiz1002@ hotmail． com。* 通讯作者，副教授，博士，从事营养保健研究，E-
mail: hkf1979@163． com。
收稿日期 2011-01-21 修回日期 2011-02-28
651 Agricultural Science ＆ Technology Vol． 12，No．1，2011