应用Biolog方法对两种转基因棉花及其亲本非转基因棉花根际土壤微生物的单一碳源利用水平进行了比较分析，探讨转基因棉花种植对其根际土壤微生物群落功能多样性的影响.结果表明：与非转基因亲本相比，在苗期、蕾期、吐絮期、衰老期转基因棉花种植对根际土壤微生物群落碳源利用能力、Shannon功能多样性指数和均匀度指数的影响均不显著，而在花铃期根际土壤微生物群落碳源利用能力和Shannon功能多样性指数显著降低.主成分分析表明，花铃期转基因棉花与非转基因棉花根际土壤微生物碳源利用在两主成分轴上的分异较大，碳源利用模式差异显著.
By the method of Biolog, a comparative study was made on the utilization level of single carbon source by the microbes in the rhizosphere soils of two
transgenic cottons and their parents, aimed to approach the effects of planting transgenic cotton on the functional diversity of rhizosphere soil microbial community. Compared with planting non-transgenic cotton, planting transgenic cotton had less effects on the carbon sourceng utilization ability, Shannon functional diversity index, and evenness index of rhizosphere soil microbes at seedling, squaring, boll-opening, and senescence stages, but decreased the carbon source utilization ability and functional diversity index at flowering and boll-forming stage significantly. Principal component analysis (PCA) indicated that there existed greater differentiation in the carbon source utilization by the microbes in rhizosphere soils of non-transgenic and transgenic cottons at flowering and boll-forming stage, suggesting the significant difference in the carbon source utilization pattern of the microbes at this stage.