Abstract:The study on the soil quality and bacterial diversity under 8-30 years old exotic Larix kaempferi, native Pinus tabulaeformis, and secondary deciduous broadleaf forest stands in Xiaolongshan Mountains of Gansu, Northwest China showed that the soil pH under different forest stands had no distinct variation, but soil moisture content was increased with increasing age of forest stands. Soil organic matter and nitrogen contents were the highest under secondary deciduous forest, followed by under L. kaermpferi, and P.tabulaeformis. However, the soils under different ages of forest stands had no obvious variations in their organic matter and nitrogen contents,suggesting that tree species was the main factor affecting soil quality. Compared with P. tabulaeformis, exotic L. kaempferi could significantly increase soil organic matter and nitrogen contents. PCR-DGGE banding patterns suggested that the soil under secondary deciduous broadleaf forest had the highest bacterial diversity, followed by under L. kaempferi, and P. tabulaeformis. The sequenced DGGE bands were classified into three bacterial groups, i.e., Proteobacteria, Cytophaga-Flavobacterium-Bacteroides, and high G+C content gram-positive type, among which, Proteobacteria occurred most frequently. Further detailed analyses suggested that the soil bacterial compositions under exotic Larix stands were more similar to each other than those under pine and secondary deciduous broadleaf forests. It was concluded that exotic L. kaempferi induced the changes of microbial diversity in the forest soils of this region.