---

作 者 ：李钧敏;金则新

期 刊 ：应用生态学报 2006年 17卷 02期 页码：305-308

关键词：子；

摘 要 ：

通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后，分离得到30株银离子抗性细菌，最大硝酸银耐受量可达80 mg·ml^-1.对这些细菌进行质粒抽提与鉴定，质粒检出率为76.67％.40 mmol·L^-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75％；而350 μg· ml^-1的吖啶橙对HAg4细菌质粒的消除率只有77.78％.细菌质粒与银离子抗性密切相关.

Abstract:

Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag⁺ solution,thirty bacteria strains with silver (Ag⁺)resistance were isolated,among which,the highest Ag^+_-resistant concentration was 80 mg·ml^-1.The plasmids in these bacteria were extracted,with the detection rate of 76.67％.The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol·L^-1 sodium benzoate,and 77.78% by 350 μg·ml^-1 acridine orange.It was suggested that the Ag⁺ -resistance of bacteria was highly correlated with their plasmids.

---