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作 者 ：丁德贵^1,2;樊美珍¹;孟艳琼¹;李增智¹;夏成润¹

期 刊 ：应用生态学报 2007年 18卷 03期 页码：564-568

关键词：子；

摘 要 ：采用Ⅰ型内含子技术对应用球孢白僵菌无纺布菌条持续控制松褐天牛的防治效果进行了分子生物学评价.结果表明: 采用常规手段评价防治效果，两个处理区的防治效果分别为19.5%和10.8%; 而采用分子手段进行评价，防治效果分别为14.2%和11%.证明常规评价以死亡率或侵染率来计算微生物防治效果，难以排除当地微生物区系中的土著菌株所造成的侵染死亡，而采用分子生物学手段进行防治效果评价可从一定程度上消除土著菌株的干扰，较准确地反映人工放菌的防治效果.

Abstract:By using molecular marker of group Ⅰ intron, a field evaluation was made on the efficacy
of sustainable control of Monochamus alternatus by non-woven fabric bands impregnated with
Beauveria bassiana. The results showed that the control efficacy in two treated plots was 19.5% and
10.8%, and 14.2% and 11% when evaluated with conventional and molecular evaluation methods,
respectively, suggesting that the conventional method which evaluated the control efficacy in terms
of microbes mortality or infection rate couldn’t distinguish the efficacies caused by released
biocontrol agents and indigenous pathogens, while molecular method could avoid the interference of
indigenous pathogens and give an objective and reasonable evaluation on the efficacy of microbial
control.

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