全 文 ：Journal of Forestry Research, 16(2): 113−116 (2005) 113


Imbibitions, energy test and accelerated ageing in primed and non-primed
seeds of Peltophorum dubium

LI Lei-hong1, ZHANG Wan-li2*, ZU Yuan-gang1, SONIA Perez3
1 Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China
2 East China Normal University, Shanghai 200062, China
3 Federal University of Sao Carlos, Sao Carlos 13565-905, Brazil

Abstract: Peltophorum dubium seeds were set to imbibe with four treatments, soaked with solution Captan 0.2% under 10 and 27 ºC,
PEG 6000 -1.0 MPa under 10 and 27 ºC. For each treatment there were four replicates with 40 seeds incubated in 9-cm Petri dishes with
double filter paper moistened with testing solution. The imbibition curves showed that the final weight increase were from 70% to 150% in
the treatments when imbibition entered a lag phase. Seeds were tested for effects on germination of five treatments: control group
(nonprimed), primed with PEG6000 -1.0 MPa at 10 and 27 ºC, primed with Captan 0.2% at 10 and 27 ºC. For each treatment, there were
three sub-treatments: seeds were soaked in distilled water for 12, 24 and 36h before the energy test. Germination percentages of nonprimed
seeds and primed in PEG 27 ºC soaked in distilled water during 12 h were the highest, reaching 100%. The lowest germination percentage
occurred primed seeds with PEG6000 27 ºC and soaked in distilled water during 36 h, which was only 52%. Germination mean time of
primed seeds in PEG at 10 ºC, soaked 24 h was 1.08 days, mean time of primed seeds in PEG at 27 ºC soaked 12 h was 2.42 days.
Accelerated ageing results showed low or no germination after ageing 72 h. Control group had a higher germination percentage and seeds
were more resistant to deterioration than those in primed groups, both in Petri dish (27 ºC) and vermiculate (room temperature).
Keywords: Peltophorum dubium; Seeds; Imbibition; Priming; Germination; Ageing
CLC number: S722.14 Document code: A Article ID: 1007-662X(2005)02-0113-04



Introduction

Peltophorum dubium (Spreng) Taub is a perennial wood
species, native to Brazilian semi-deciduous forests, about 15–25
m in height and 50–70 cm in diameter, with high quality wood
products. This species is listed in endangered species. It is a fast
growing and sturdy species that dose well in sunny climate and
often used for reforestation of degraded areas. It is also
recommended for street planting, for it provides excellent shade
(Lorenzi 1992).
The uptake of water by seeds is an essential initial step toward
germination. Under optimal supply conditions, the water uptake
by seeds is triphasic. Phase I, or imbibition, is largely a
consequence of matric forces, and water uptake occurs regardless
of whether the seed is dormant or undormant, viable or nonviable.
This phase is marked by three characteristics: 1) a sharp front
separating wet and dry portions of the seed, 2) continued
swelling as water reaches new regions, and 3) an increase in
water content of the wetted area. Phase II is the lag phase of
water uptake and Phase III is concurrent with radicle elongation
and only germination seeds enter (Bewley & Black, 1986).
Seeds treated with osmotic when introduced to water, these
seeds, which are nearly all at the same germination stage, show
rapid, almost synchronous radicle emergence. Advantages of this
treatment--called osmotic priming--are that treated seeds take

Foundation item: This work is supported by CAPES, Brazil. Open
research laboratory of forest plant ecology, Northeast Forestry University
and The States tenth five-year 211 Project-supported key academic
discipline program of ECNU。
Biography: LI Lei-hong (1971-), Engineer, Northeast Forestry University,
Harbin 150040, P. R. China E-mail: leeleihong@yahoo.com.cn
Received date：2005-3-28
Responsible editor: Chai Ruihai
*Corresponding author: wlzhang@bio.ecnu.edu.cn
less time to germinate (Bewley & Black 1986). The use of
PEG6000 priming for limited imbibition appears to hold the
most potential for inducing early and synchronous germination
of seeds. Primed seed with salt solution was effective in
increasing seedling emergence and for reducing the time of
emergence in summer greenhouse studies (Yoon et al. 1997).
Ageing in all organisms is the sum total of the deterioration
processes that eventually lead to death. For most plant species,
the so-called orthodox species with respect to the response of
their seeds to storage conditions, the time taken for half of the
population of seeds to become incapable of germinating is much
shorter at a higher moisture content and/or temperature.
The ageing of seeds is indicated by delayed germination,
slower growth and increased susceptibility to environmental
stress, eventually leading to loss of viability (Byrd and Delouche
1971; McDonald & Nelson 1986). Accelerated ageing of seeds at
elevated relative humidity and high temperature lost germination
and seed viability and vigor. Some reports (Deraman et al. 1987;
Tarquis and Bradford, 1992; Smok et al. 1993; Corbineau et al.
1994) have shown that primed seeds are more sensitive to
accelerated ageing than nonprimed ones while other finds
(Dearman et al. 1986) have demonstrated a protective effect of
priming on subsequent tolerance to ageing.
The aim of this work is to show imbibition curves of P.
dubium seeds under different temperatures and solutions as well
as germination ability and resistance to ageing.

Materials and methods

Biological material
The seeds of P. dubium were provided by Copersucar, São
Paulo state, Brazil. These seeds are orthodox and stored in
hermetic condition at 5ºC throughout the experiments. All the
seeds for experiment were previously scarified with 98% sulfuric
acid for 15 min to overcome mechanical dormancy (Perez et al
LI Lei-hong et al. 114
1999) and washed in tap and distilled water, dried under room
temperature.

Basic experimental procedure
The Petri dishes of 9 cm or 15 cm in diameter, with double
filter paper in each, were sterilized 2 h at 150ºC to minimize the
effect of contamination on the seeds during the tests. Vermiculite
was also sterilized for the test. PEG6000 solutions were prepared
according to the osmotic potential described in Villela et al.
(1991). Testing seeds were primed in Captan 0.2% solution for 4
h at 27 ºC in an aluminum box with double filter paper
moistened with Captan 0.2% solution, initial weight was noted
down before priming. After priming the seeds were washed and
dried, keeping in refrigerator at 27 ºC until the final weight
returned to the initial weight, and then the seeds were ready for
the test. Priming time in Captan solution at 10 ºC was 10 h, 24 h
in PEG6000 both at10 ºC and 27 ºC, 24 h in KNO3.

Imbibition
There were four treatments for the imbibition: seed tested with
Captan 0.2% at 10 ºC and 27 ºC, PEG at 10 ºC and 27 ºC ,with
four replicates for each treatment. Ten seeds were incubated in
each replicate 9-cm Petri dish with double filter paper soaked
with the testing solution of 4–6 ml. Initial dry weight of the seeds
was recorded for each replicate. Final weight was recorded to
compare the initial weight every 2 h in the first 24 h during the
imbibition, every 6 h in the next 48 h and then every 12 h until
the radicle emergence ending the imbibition test. Standard errors
should be controlled at 5% among replicates within treatments.
For the treatment of Captan 27 ºC, it is necessary to record the
final weight every hour from 12 h to 24 h during the imbibition
test because of the fast water uptaking. New filter paper would
substitute the old paper when its color turned yellow. Fungus
was removed during the experiment.

Energy test
All the seeds were set for the experiment in Petri dishes of 9
cm diameter, five replicates with 50 seeds after soaking in
distilled water during 12, 24 and 36 h.
Germination was observed every 24 h. Germinated seeds with
radicle protrusion ≥ 2 mm and with position geotropic curve
were eliminated, as well as the dead seeds or those without any
condition of germination. The tests were considered to be
finished when all seeds had germinated or when they no longer
had the possibility to germinate. Final data included total
germination (G), mean time (t), germination speed (r) (Labouriau,
1983). Primed seeds and nonprimed seeds were also planted in
the experimental garden for field performance. For each group
five replicates were made with 100 seeds. Daily observation of
seedling emergence ≥ 2 cm was noted. Four weeks later,
seedlings were cut for analysis. Final germination percentage and
above ground dry matter were collected.

Accelerated ageing
Five groups were chosen for accelerating ageing test: control
group, primed in Captan at 10 ºC and 27 ºC, primed in PEG at 10
ºC and 27 ºC. For each group, 600 seeds were accelerated ageing
in the ageing camera with 100% humidity at 43–45 ºC for 24, 48
and 72 h. Seeds were taken out of the camera and fungus was
removed before setting to germinate in the incubator (100 seeds)
at 27 ºC and in the vermiculite (100 seeds) in the laboratory.
Daily observation of the germination was necessary and fungus
in the seed lots need to be removed.

Statistical analysis
Data are tested using ANOVA (Sokal and Rohlf, 1980).

Results and discussion

Imbibition
Imbibition tests of P. dubium seeds in different solutions
under different temperatures showed the difference on the
process of water uptaking.
Results show that the sharp increase of the weight of seeds
with solution Captan at 10 ºC in the first 24 h, while the sharp
curve increase happened on the seeds with Captan 0.2% at 27 ºC
in the first 12 h. And imbibition curves of seeds with PEG -1.0
MPa at 10 and 27 ºC were sharp in the first 24 h. Then each
curve became to fluctuate around its stable line. Radicle emission
of seeds with Captan 27 ºC was occurred after 24 h of the
imbibition.
This imbibition experiment of P. dubium finished when the
radicle emerged, so the curves show the first and second phases
of water uptaking. The imbibition of seeds is an essential and
initial step toward germination.
From Fig. 1, the final weight was about 250% of the initial
weight of Captan 10 ºC, 240% of the initial weight of Captan
27oC, 180% of the initial weight of PEG 10oC, and 170% of the
initial weight of PEG 27oC. Then all of the imbibition entered a
lag phase and the curves became stable after the sharp increase
of the seeds weight. Imbibition in Captan 27oC was much faster
than those in PEG solutions due to the water stress on the seeds.

0 10 20 30 40 50 60 70 80 90 100 110
0
50
100
150
200
250
Pe
rc
en
ta
ge
(%
)
Time(hour)
B
C
D
E
Fig.1 Imbibition curves of Peltophorum dubium in different
solutions under different temperatures.
B: Captan 10℃, C: Captan 27℃, D: PEG 10℃, E: PEG27℃.

Germination
The germination percentage and speed of the seeds in different
treatments and soaked in distilled water were shown in Table 1.
Seeds soaked in water for 12 h in control group had the highest
germination (100%) and germination speed (0.95 day-1). Seeds
primed in PEG 10 ºC and 27 ºC and soaked in water 12 h also
have full germination result (100%). For seeds in control group
soaked in water for 24 h, each group had different germination
speed. Seeds primed in PEG 27oC soaked in water 12 h had the
25
20
15
10
25
10 20 5 60 70 80 90 10 1
Journal of Forestry Research, 16(2): 113−116 (2005) 115
slowest germination speed (0.45 day-1) and seeds primed in PEG
10 ºC soaked during 36 h had the quickest germination speed
(0.975 day-1). There was no significant difference in germination
percentage between the primed and nonprimed seeds except
those that were primed in PEG 27 ºC and soaked in water during
24 and 36 h and in Captan 10 ºC and soaked during 36 h.
Germination percentage of seeds primed in PEG 27 ºC soaked in
water during 36 h had the lowest value and showed significant
difference from other groups. Seeds from control group and
primed in Captan 27 ºC soaked 36 h did not show much
difference from other groups except seeds primed in PEG 27 ºC
soaked 36 h. There was no significant difference in germination
percentage between treatments of seeds primed in PEG 27 ºC
soaked 24 and 36 h, and in Captan 10 ºC soaked 36 h.

Table 1. Mean germination percentage and speed for P. dubium
seeds of nonprimed (control) or primed in Captan (10 or 27 ºC),
PEG (-1.0 Mpa) (10 or 27 ºC) soaked in water during 12, 24, 36 h.
Treatment Germination
Percentage (%)*
Germination
Speed(days-1)
Control (12 h)
Primed in Captan 10(12 h)
Primed in Captan 27(12 h)
Primed in PEG 10(12 h)
Primed in PEG 27(12 h)
Control (24 h)
Primed in Captan 10(24 h)
Primed in Captan 27(24 h)
Primed in PEG 10(24 h)
Primed in PEG 27(24 h)
Control(36 h)
Primed in Captan 10(36 h)
Primed in Captan 27(36 h)
Primed in PEG 10(36 h)
Primed in PEG 27(36 h)
100 A
90 A
92.5 A
100 A
100 A
100 A
90 A
97.5 A
97.5 A
65 BC
82.5 AB
75 BC
80 AB
87.5 A
55 C
0.95 A
0.675 BC
0.575 BC
0.725 BC
0.45 C
0.775 AB
0.675 BC
0.825 AB
0.95 A
0.55 BC
0.775 A
0.525 C
0.575 BC
0.975 A
0.825 AB


dms=20.79
F=11.13
Fc=2.16
dms=0.21
F=16.357
Fc=2.16
* means followed by the same letter on the same column data differ at
P<0.05.

Table 1 also gives information about germination speed from
seeds in all the groups. There was no significant difference in
seed germination speed between control groups, groups primed
in Captan (27 ºC) and PEG (10 ºC) which were soaked 24 h,
PEG (10 ºC) and 27 ºC soaked 36 h, but these groups had
difference from the other groups. The slowest germination speed,
0.45 day-1, occurred on seeds primed in PEG (27 ºC) soaked 12 h,
but its germination percentage was also 100.
Seeds soaked 36 h from control group had lowest germination
percentage For seeds primed in Captan (10 ºC), higher
germination percentage and speed occurred from seeds soaked
during 12 and 24 h. For seeds primed in Captan (27 ºC), 24-h
soaking seeds had a higher germination percentage and
germination speed. For seeds primed in PEG (10 ºC), 36-h
soaking seeds had a lower germination percentage. For seeds
primed in PEG (27 ºC), 12-h soaking seeds had a higher
germination percentage but lower germination speed.
Seeds soaked 12 h showed no significant difference in
germination percentage, but much difference in germination
speed, and control group had the highest germination speed and
showed significant difference from others.
For seeds soaked 24 h, there were no significant difference in
germination percentage between the experimental groups, except
PEG (27 ºC) which had the lowest germination percentage, but
they had significant difference in germination speed, highest
value for treatment of PEG (10 ºC) and the lowest value for
treatment of PEG (27 ºC).
For 36-h soaked seeds, the seeds with different treatments of
showed significant difference in both germination percentage
and germination speeds. Seeds treated with PEG (10 ºC) had the
highest germination percentage and germination speed, seeds
treated with PEG (27 ºC) had the lowest germination percentage,
and seeds treated with Captan (10 ºC) had the lowest value of
germination speed.

Field performance
Field performance of primed seeds and nonprimed seeds was
observed on germination percentage and above-ground dry
matter of 4-week-old seedlings. All the experimental groups
showed no significant difference in both germination percentage
and dry matter (Table 2). Nonprimed seeds had the highest
germination percentage (96.25%) and individual dry matter
(0.061 g). Seeds primed in Captan (27 ºC) had the lowest
germination percentage (87.5%) and PEG (10 ºC) had the lowest
value of dry matter (0.051g).

Table 2. Germination percentage and above ground dry matter of P.
dubium seeds of different treatments in field performance
Treatment Germination
Percentage (%)
Individual
dry matter(g)
Control
Primed in Captan(10oC)
Primed in Captan(27oC)
Primed in PEG (10oC)
Primed in PEG 27(oC)
96.25 A
92.5 A
87.5 A
88.75 A
95 A
0.061 A
0.055 A
0.054 A
0.051 A
0.056 A
Dms=9.57
F=3.03261
Fc=3.8
Dms=0.01
F=2.77
Fc=3.8


Accelerated ageing
Accelerated ageing on the germination for nonprimed and
primed seeds was conducted in both incubator at 27 ºC and
vermiculite at room temperature. Nonprimed seeds after 24 h
ageing showed highest germination percentage at 27 ºC and at
room temperature (Table 3). There was no significant difference
in germination percentage between the primed groups ageing 24
h at room temperature except the seeds primed in PEG (10 ºC).
No significant difference was observed among the groups at
room temperature except control group ageing 24 h. All the
seeds after aging 24, 48 and 72 h showed very low germination
percentage were (Table 3) and had a trend of decrease in
germination percentage with the increase of ageing period. After
ageing 72 h, almost no germination occurred at 27oC and at room
temperature. Seeds of control group were found more resistant to
ageing deterioration during the test, still the germination
percentage was only 20% at 27 ºC after ageing 24 h and as lower
as 5% after ageing 48 h. Seeds of control group had only 6%
germination at room temperature after ageing 24 h..
LI Lei-hong et al. 116
Table 3. Germination percentage of primed and nonprimed P.
dubium seeds in incubator and vermiculite after accelerated
ageing.
Germination percentage (%) Ageing
Time
(h)
Treatments Petri dish
(27 ºC)
Vermiculite
(room
temperature)
24
Control
Primed in Captan(10oC)
Primed inCaptan(27oC)
Primed in PEG(10oC)
Primed in PEG(27oC)
20 A
5 B
4 B
3 B
4 B
6 A
3 AB
2 AB
3 AB
1 B
48
Control
Primed in Captan(10oC)
Primed inCaptan(27oC)
Primed in PEG(10oC)
Primed in PEG(27oC)
5 B
2 B
1 B
2 B
4 B
2 AB
1 B
1 B
0 B
1 B
72
Control
Primed in Captan(10oC)
Primed in Captan(27oC)
Primed in PEG(10oC)
Primed in PEG(27oC)
1 B
1 B
0 B
0 B
1 B
0 B
0 B
0 B
0 B
0 B
Dms=5.91
F=17.4801
Fc=2.12
Dms=4.48
F=3.75918
Fc=2.12

However, primed P. dubium seeds are more sensitive to
accelerate ageing than the nom-primed seed. The same
phenomenon was observed with seeds of carrot (Dearman et al.
1987 and sunflower (Smok et al. 1993) and leek (Corbineau et al.
1994).

Conclusions

The imbibition process of P. dubium was affected by both
solution concentrations and temperatures. The lowest
temperature and osmotic potential decreased the imbibition rate.
Germination in the incubator at 27 ºC and field performance
showed the nonprimed seeds of P. dubium had a higher
germination percentage and above ground individual dry matter
compared with those physiologically primed in solutions Captan
0.2% and osmoconditioned in solutions PEG6000 -1.0 MPa. It is
difficult to conclude that osmotic priming with PEG 6000 or
priming with Captan 0.2% had a better effect on the germination
of P. dubium seeds, at least at the early stage, priming did not
show evidence to improve the seedling’s growth. Probably more
similar experiments need to be conducted on this area to compare
those attempts on some vegetable species and tree species.

References

Bewley, J. Derek & Black Mmichael. 1986. Seeds: Physiology of
development and germination [M]. New York and London: Plenum Press,
pp. 115, 110–114, 119.
Byrd, H.W. and Delouche, J.C. 1971. Deterioration of soybean seed in
soybean seed in storage [J]. Proc. Assoc. Official Seed Anal., 61: 41–57.
Corbineau F. et al. 1994. Germinability of leek seeds and its improvement by
osmopriming [J]. Acta Horticulturae, 371:45–52.
Dearman, J., et al. 1987. Effects of osmotic priming and ageing on the
germination and emergence of carrot and leek seed [J]. Annals of Applied
Biology. 111: 717–722.
Heydecker, W., Higgins, J. & Gulliver, R. L. 1973. Accelerated germination
by osmotic seed treatment [J]. Nature, London, 246: 42–44,.
Labouriau, L.G. 1983. A germinacao das sementes. Secretaria geral da O.E.A.,
Washington
Lorenzi, H. 1992. Árvores brasileiras: Manual de identificaçao e cultivo de
plantas arbóreas nativas do Brasil [M]. Nova Odessa: Editora Plantarum,
pp.161,
McDonald, M.B., 1996. A review and evaluation of seed vigor tests [J]. Proc.
Assoc. Official Seed Anal., 65:19–39.
Smok, M.A. et al. 1993. Effects of osmotic treatment on sunflower seed
germination in relation with temperature and oxygen. Fourth International
Workshop on Seeds. Vol.3:1033–1038.
Sokal, R.R. and Rohlf, F.J. 1980. An experiment in taxonomic judgement [J].
Systematic Botany, 5: 341–365
Tarquis, A.M. et al. 1992. Prehydration and priming treatments that advance
germination also increase the rate of deterioration of lettuce seeds [J].
Journal of Experimental Botany,.43:307–317..
Villela, F.A. et al, 1991.Tabela de potencial osmotico em funcao da
concentracao de polietileno glicol 6000 e da temperature [C]. In: Pesquisa
Agropecuaria Brasileira, Brasilia, v.26 n.11/12, p 1957-1968.


Chinese Abstracts 2
为研究白桦雌花序花期基因表达情况，以两天为间隔对
其进行取样。基于SMART策略，通过RT-PCR，将源自最后
时期样品的cDNA作为Driver cDNA，源自其他时期样品的
cDNA作为Tester cDNA，构建抑制性消减文库。EST序列经
blastX分析表明，该文库中的基因大致可以归为五类，分别同
代谢、物质运输和信号转导、细胞周期、胁迫反应及调控相
关。本文对基因表达同发育的关系做了探讨。图4参25。
关键词：白桦；抑制性消减杂交；SMART
CLC number: S792.153 Document code: A
Article ID: 1007-662X(2005)02-0097-04

05–02–005
不同土壤层次和土壤水分对章古台樟子松固沙林土壤 N矿化
过程的影响/陈伏生（中国科学院沈阳应用生态研究所大青沟
沙地生态实验站，沈阳市 110016；中国科学院研究生院, 北
京 10003，中国），曾德慧，SINGH Anand Narain （中国科
学院沈阳应用生态研究所大青沟沙地生态实验站，沈阳市
110016，中国），陈广生(中国科学院沈阳应用生态研究所大
青沟沙地生态实验站，沈阳市 110016；School of Forestry and
Wildlife Sciences, Auburn University, AL 36830, USA.)
//Journal of Forestry Research. −2005, 16 (2): 101–104.
选择章古台地区三块典型樟子松（Pinus sylvestris var.
mongolica）人工固沙林为研究对象，采用实验室好氧培养法
测定了不同土壤层次和在不同水分条件下的 N矿化过程。结
果表明：土壤 0-60 cm 层 N净矿化速率垂直变化范围为
1.06–7.52 mg·kg-1·month-1；土壤层次和含水量及其交互作用对
土壤 N净矿化速率的影响均达到差异显著（P<0.05）；净矿
化速率随着土壤层次的加深而明显下降，0-15 cm层占总净矿
化量的 60.52%；半饱和与饱和含水量处理差异不显著，但均
高于不加水处理。为此，在半干旱地区必须进一步加强开展
调控生态系统 N矿化、循环及其收支平衡影响因素的研究。
图 1表 4参 20。
关键词：实验室好氧培养法；N矿化；管理措施；固沙林；
半干旱地区
CLC number: S791.253; S714.5 Document code: A
Article ID: 1007–662X(2005)02–0101–04

05–02–006
中国西南干旱河谷区植物多样性边界影响域的两种判定方法
/李丽光(中国科学院沈阳应用生态研究所，沈阳 110016，中
国；中国科学院研究生院，北京 100039，中国)，何兴元，李
秀珍(中国科学院沈阳应用生态研究所，沈阳 110016，中国)，
问青春，赵永华(中国科学院沈阳应用生态研究所，沈阳
110016，中国；中国科学院研究生院，北京 100039，中国) //
Journal of Forestry Research. −2005, 16(2): 105–108.
研究了中国西南岷江上游干旱河谷区不同类型景观边界
的影响域。共选取三种类型的边界。以植物多样性为基础，
分别采用主成分分析法和移动窗口法对边界的影响域进行分
析。结果表明, 5条样带中，主成分分析法可以判定 3条样带
的影响域，而移动窗口法可以判定 4条。两种方法均可判定
边界的影响域，并且影响域均在距边界 50m内。在林地样带
两种方法可以得出相似的结论，但在花椒地样带中所得结论
不一致。两种方法比较，移动窗口法更成功些。虽然两种方
法各有利弊，但它们都是刻画边界动态的有力工具。图 8表
2参 22.
关键词：边界；移动窗口法；主成分分析；影响域；景观
CLC number: S711 Document Code: A
Article ID: 1007–662X(2005)02–0105–04

05–02–007
紫色土人工林林下植物区系与群落外貌的恢复过程/于占源
（中科院沈阳应用生态研究所，沈阳 110016；中国科学院研
究生院，北京 100039；福建农林大学，南平 353001），岳
永杰（四川省林科院，成都 610081），郭剑芬，陈光水（福
建师范大学，福州 350007），谢锦升，何宗明（福建农林大
学，南平 353001），杨玉盛（福建师范大学，福州 350007）
//Journal of Forestry Research.–2005, 16(2): 109–112.
采用时空代换法，以福建省宁化县严重退化紫色土人工
林生态系统为对象，按侵蚀强度由强到弱选取 4种生态恢复
措施Ⅰ、Ⅱ、Ⅲ、Ⅳ，研究了植物多样性与生态系统过程的
关系。结果表明，该紫色土退化生态系统的植物出现 43科 78
属 86种，种子植物属 15个分布类型中出现 12个。沿恢复梯
度，植物的生活型谱逐渐丰富，种子植物属各分布区类型出
现的属数趋于增加，各种叶特征所对应的植物属数依次增多。
可见，生态恢复过程中的植物区系与群落外貌越来越复杂和
多样化，该紫色土生态系统渐趋稳定。表 6参 14。
关键词：紫色土；植物区系；群落外貌；生物多样性；生态
恢复过程
CLC number: S718.55 Document code: A
Article ID: 1007-662X(2005)02-0109-04

05–02–008
预处理对巴西盾柱木种子吸涨、活力测试及其加速老化的影
响/李雷鸿(东北林业大学森林植物生态学开放研究实验室，哈
尔滨市 150040)，张万里(华东师范大学生命科学学院，上海
市 200062)，祖元刚(东北林业大学森林植物生态学开放研究
实验室，哈尔滨市 150040)；SONIA Perez （Soni Perez
(Botanical Department, Federal University of Sao Carlos, Sao
Carlos 13565-905, Brazil) // Journal of Forestry Research .−2005,
16(2): 113−116.
对巴西盾柱木（Peltophorum dubium）种子进行了 4种处
理的吸胀实验，即将种子分别置于 10℃和 27℃下 0.2% Captan
溶液中和-1.0MPa的 PEG6000溶液中，每个处理有四个重复，
40粒种子，置于培养皿中浸满实验溶液的双层过滤纸上，覆
以透气良好的薄膜。种子在实验前均用 98%的浓硫酸处理 15
分钟以打破机械休眠。吸胀曲线显示，种子在吸胀过程中最
终重量的增加值为 70%-150%，以后进入一个停滞期。在 5
种处理下（对照组，PEG6000溶液中 10℃和 27℃下预处理，
Captan溶液中 10℃和 27℃下预处理），对种子萌发所受到的
影响进行了实验。每一个处理分别进行 3个次级处理，即在
实验前，将种子用蒸馏水分别浸泡 12、24和 36小时。萌发
率最高的是经过蒸馏水浸泡 12小时对照组种子和 PEG 27℃
组的种子，萌发率达到 100%；萌发率最低的是蒸馏水中浸泡
36小时的 PEG 27℃组的种子，萌发率为 52%；经过蒸馏水
浸泡 24小时的 PEG 10℃组种子的平均萌发时间为 1.08天。
蒸馏水中浸泡 12小时的 PEG 27℃组种子平均萌发时间为
2.42天，其它处理的萌发时间值介于两者之间。对预处理和
未经预处理种子通过加速老化实验，对其活力和生存力进行
了测试。经 72小时加速老化，种子萌发率低或没有萌发力。
对照组种子在培养皿（27℃）和室温条件下蛭石中的萌发率
都比处理组高，表现了对老化更强的抗性。图 1表 3参 12。
关键词：巴西盾柱木；吸涨；预处理；萌发；老化
CLC number: S722.14 Document code: A
Article ID: 1007-662X(2005)02-0113-04

05–02–009
结缕草构件种群年龄结构与动态的研究/代保清(中国科学院
沈阳应用生态研究所，沈阳 110016)，王艳（沈阳师范大学化