免费文献传递   相关文献

长穗柽柳种子形态特征与萌发特性初步研究(英文)



全 文 :ResearchontheMorphologicalCharacterand
GerminationCharacteristicsofTamarix
elongataLedeb.Seeds
YUJun1 , JIANGXi2 , BAIBao-wei3 , JIAOPei-pei4, 5 , PANGXin-an4, 5*
1.DepartmentofScienceandTechnology, TarimUniversity, Alar843300;2.ColegeofPlantScienceandTechnology, TarimUniversity,
Alar843300;3.ColegeofLifeScience, TarimUniversity, Alar843300;4.KeyLaboratoryofProtectionandUtilizationofBiologicalRe-
sourcesinTarimBasinofXinjiangProductionandConstructionCorps-CultivationBaseofStateKeyLaboratory, Alar843300;5.Biological
TechnologyResearchandDevelopmentCenter, TarimUniversity, Alar843300
Abstract [ Objective] TheaimwastostudythegerminationbehaviorofTamarixelongataLedeb.undernaturalconditions.[Method] Byusing
T.elongataastheexperimentalmaterials, theseedmorphologyandgerminationcharacteristicswereobservedandmeasured.[ Result] ①T.
elongataseedwas1 011.96μminlengthand399.94μminwidth, andthethousand-seedweightwas38.66mg;②thegerminationrate, germi-
nationenergy, germinationindexandgerminationcoeficientofT.elongataseedswere95.56%, 46.67%, 20.25and56.86respectively;③
therewerenosignificantdiferencesinmorphologyandgerminationcharacteristicsamongthethreediferentgeographicalpopulations.[ Con-
clusion] ThepresentresearchwilprovidereferencefortheintroductionandbreedingofT.elongataaswelastheprotectionofsanddunevegetation.
Keywords TamatixelongataLedeb.;Seed;Morphology;Germination
Received:November8, 2010  Accepted:December19, 2010
SupportedbyaGrantfromtheNationalNaturalScienceFoundation
ofChina(30670133).
*Correspondingauthor.E-mail:1517504814@qq.com
  TamarixelongataLedeb.belongingtoTamarixspp.,
Tamaricaceae[ 1] isakindofshruborsmalarbor, 1 -5 min
height;besides, itflowersfrom April-Mayandfruitsfrom
May-June[ 2].T.elongatawithmanyadvantagesincluding
light-loving, drought-resistanceandsaline-alkaliresistanceis
akindofgoodtreespeciesforwindbreakandsandfixation,
aforestationandgreening, soilandwaterconservation[ 3].
Therelatedresearchonmorphologicalanatomy, community
ecology, physiologicalecologyandplantchemistryofT.elon-
gatahavebeencarriedoutalovertheword[4-10] ;however,
theintroductionandcultivation, breedingofseedsourceof
T.elongatahavebeenrarelyreported[ 11-13] , whichneedsto
befurtherstudied.Inthepresentresearch, byusingT.elon-
gataastheexperimentalmaterials, theseedmorphologyand
germinationcharacteristicswereobservedtounderstandthe
germinationbehaviorofT.elongataundernaturalconditions,
toprovidereferencefortheintroductionandbreedingof
T.elongataaswelastheprotectionofsanddunevegetation.
MaterialsandMethods
Materials
T.elongataseedswerecolectedfromthediferentgeo-
graphicalpopulationsinAlarCitylocatedinupperreachesof
TarimRiverinnorthernpartofTarim Basin;maturefruits
werepickedandair-dried, andpreservedatroomtemperature
forlateruse.
Methods
Determinationofseedsizeandweight Fulmatureseeds
ofT.elongata(ofwhichtheepidermalhairhadbeenre-
moved)wereselectedwithnakedeye;thelengthandwidth
of30 seedsweremeasuredunderstereomicroscopeandthen
averagedrespectively.Thethousand-seedweightwasmeas-
uredbyusinganalythicalbalancewith0.000 1 accuracy,
whichwasrepeatedfor3 times, andtheaveragevaluewas
recoded.
Seedgerminationexperiment FulmatureseedsofT.elon-
gata(withepidermalhair)wereselectedwithnakedeye;then
every30seedswereuniformlyplacedinaPetridishes(diame-
terof90 mm)laidwithmoistfilterpaper, with3 repetitions;
thedisheswerelabeledandplacedundernaturalconditions
(room temperature, naturallight, distiledwater)forseed
germination, andobservedeveryday;thedailygerminated
seedswererecordedaccordingtothegerminationstandard
thattheradiclebreakthroughtheseedcoat.Appropriatedis-
tiledwaterwasaddedsometimestokeepthefilterpaper
moistuntiltheendofseedgerminationexperiment.
Dataprocessing
Thegerminationrate, germinationenergy, germination
indexandgerminationcoefficientwerecalculatedaccordingto
thefolowingformulas:germinationrate=(Totalnumberofthe
germinatedseedstiltheendoftheexperiment/totalnumberof
thetestedseeds)×100%;germinationenergy=(Numberof
normalgerminatedseedswithinthedefineddate/Totalnumber
ofthetestedseeds)×100%;germinationindex=΢(Numberof
germinatedseedsonthatday/Numberofdaysofseedgermina-
tion);germinationcoeficient=100×΢(Dailygerminatedseeds
number)/΢(Dailygerminationseednumber×Numberofdays
fromthebeginningtotheendofgermination).Thedatawere
recordedintheformofmean±standarderror.One-WayANO-
VAofExcel(2003)Softwarewasusedforthecalculationof
diferencesamongtheindexesandsignificanttest.
ResultsandAnalysis
Seedsizeandweight
T.elongataseedsfromthedifferentgeographicalpopula-
AgriculturalBasicScienceandMethodAgriculturalScience&Technology, 2010, 11(11-12):50-51, 58Copyright 2010, InformationInstituteofHAAS.Alrightsreserved.
DOI :10.16175/j.cnki.1009-4229.2010.z2.051
tionswerealsmalerandlighter.Theaveragelength, width,
aspectratio, thousandseedweightwererespectively1 011.96
μm, 399.94 μm, 2.53, 38.66mg.Besides, therewasnosig-
nificantdiferenceinseedsizeandweightamongthedifferent
populations(Table1).
Table1 ThesizeandweightofT.elongataseeds
Popula-
tions
Length
μm
Width
μm
Length-width
ratio
Thousand-seed
weight∥mg
A 1 012.13±12.73 397.97±5.70 2.55±0.02 38.64±0.14
B 1 014.29±13.19 401.20±5.98 2.53±0.01 38.36±0.21
C 1 009.45±13.57 400.66±6.10 2.52±0.01 38.99±0.15
Seedgerminationcharacteristics
AloftheT.elongataseedsfromthedifferentgeograph-
icalpopulationscouldgerminate.Theaveragegermination
rate, germinationenergy, germinationindexandgermination
coeficientwere95.56%, 46.67%, 20.25and56.86, respec-
tively.Therewasnosignificantdifferenceinthegermination
indicesamongthediferentpopulations.Theseedgermination
indexeswereshowninTable2.
Table2 ThegerminationindexesofT.elongataseeds
Popula-
tion
Germination
rate∥%
Germination
energy∥%
Germination
index
Germination
coefficient
A 95.56±1.11 46.67±1.92 20.22±0.44 56.59±0.71
B 96.67±1.92 47.78±1.11 20.64±0.15 57.68±0.95
C 94.44±1.11 45.56±2.22 19.89±0.26 56.30±0.42
Conclusions
Seedmorphologicalcharacteristics
T.elongatafruitwascapsulewithreddishorearthyelow
pericarp, containingseveralseeds;theseedwassmaland
light, rod-like, brownorpurple, withtuftedepidermalhairsat
thetip;besides, theseedcouldfloatintheairanddisseminate
relyingonwind;therewasnosignificantdifferenceinseedmor-
phologyamongthediferentgeographicalpopulation.
Seedgerminationcharacteristics
AloftheT.elongataseedsfromthedifferentgeograph-
icalpopulationscangerminateundernaturalconditions(room
temperature, naturallight, distiledwater), andthegermina-
tionconditionswerenotharsh;thisstudyalsofoundthatthe
seedgerminationhadsomecharacteristicsincludinghigherseed
vigor, strongerseedlifeforce, rapidgerminationandtidyseed-
lings;therewasnosignificantdifferenceingerminationcharac-
teristicsamongthediferentgeographicalpopulations.
Acknowledgements
IwishtoexpressmysincereappreciationtoLIUMing-ting
whohadprovidedusefulhelpforspeciesidentificationof
Tamatixelongate.
References
[ 1] EditorialCommiteeofFloraofChina, ChineseAcademyofSci-
ences(中国科学院中国植物志编辑委员会).FloraofChina:volume
50(中国植物志:第 50卷)[ M].Beijing:SciencePress(北京:科学
出版社), 1990:146-166.
[ 2] EditorialCommiteeofDesertFloraofChina, ChineseAcademyof
Sciences(中国科学院中国沙漠植物志编委会).DesertFloraofChi-
na:volume2(中国沙漠植物志:第 2卷)[ M].Beijing:SciencePress(北京:科学出版社), 1987:370-381.
[ 3] LIUMT(刘铭庭).ResearchoncomprehensiveutilizationofTamar-
ixspp.anditsapplicationsinlargescale(柽柳属植物综合利用研究
及大面积推广应用)[ M] .Lanzhou:LanzhouUniversityPress(兰
州:兰州大学出版社), 1995.
[ 4] FENGY(冯缨), YINLK(尹林克).Studyonorgansmorphology
anditstaxonomicsignificanceofTamarixL.(柽柳属植物镜下器官
特征描述及分类学意义)[ J].AridZoneResearch(干旱区研究),
2000, 17(3):40-45.
[ 5] FANGMY(方敏彦), ZHANGM(章明), DINGP(丁品), etal.AstudyonpopulationandcommunitystructureofTamarixelongata
growinginheavysalinesoilsinthemiddlereachesoftheShule
River(疏勒河中游高盐生境长穗柽柳群落格局研究)[J].ActaPrat-
aculturaeSinica(草业学报), 2009, 18(3):20-27.
[ 6] FANGMY(方敏彦), DINGP(丁品), ZHANGDG(张德罡).Study
onpopulationspatialpatern, interspecificassociationofTamarix
elongatadistributedonsaleduneinmiddlereachesofShuleRiver
(疏勒河中游盐质沙丘分布长穗柽柳种群空间格局及种间联结性研
究)[ J] .AnimalHusbandryandFeedScience(畜牧与饲料科学),
2008, 29(6):21-23.
[ 7] DINGP(丁品).TheCharacteristicsofpopulation, communityand
clonalgrowthofTamarixelongataLedeb.(长穗柽柳(Tamarixelon-
gata)种群学、群落学及克隆生长特性研究)[D].Lanzhou:Gansu
AgriculturalUniversity(兰州:甘肃农业大学), 2007.
[ 8] QUYP(屈艳萍), KANGSZ(康绍忠), XIAGM(夏桂敏), etal.
SapfluxinthetrunksofTamarixelongatatreesplantedintherea-
chesofShiyangheRiverofGansuProvince(甘肃石羊河流域人工
种植长穗柽柳树干液流量变化规律研究)[ J].ActaBotanicaBorea-
li)-OccidentaliaSinica(西北植物学报), 2005, 25(11):2259-
2265.
[ 9] QUYP, KANGSZ, LIFS, etal.XylemsapflowsofirrigatedTam-
arixelongataLedebandtheinfluenceofenvironmentalfactorsin
thedesertregionofNorthwestChina[J].HydrologicalProcesses,
2007, 21(10):1363-1369.
[ 10] ABABAIKELIG(古丽巴哈尔.阿巴拜克力), ABBASA(阿不都拉.
阿巴斯).Studyonextractiontechnologyoftotalflavonoidscon-
tentsinTamarixelongataLdb.(新疆长穗柽柳总黄酮提取工艺研
究)[J].FoodScience(食品科学), 2007, 28(9):332-335.
[ 11] YINLK(尹林克).Theex-situprotectionandtheecologicaladaptabilityofTamarixL.inChina(柽柳属植物的生态适应性与引
种)[J].AridZoneResearch(干旱区研究), 2002, 19(3):12-
16.
[ 12] NIP(倪萍).Studyonestablishmentpopulationandecologicalef-
ficiencyofTamarixsppindisturbedareaofbothsidesofroadin
JunggarBasin(准噶尔盆地公路两侧受扰动区柽柳种群的发生和生
态效应)[D].Urumqi:Xinjianguniversity(乌鲁木齐:新疆大学),
2005.
[ 13] ZHAOXF(赵新风), ZHUYF(朱艳芬), XUHL(徐海量), etal.
Comparisonofdiasporeshape, size, andmassofmaindesertplantsinthelowerreachesofTarimRiverofXinjiang, China(塔里
木河下游主要荒漠植物繁殖体的形状、大小与质量比较)[ J].Chi-
neseJournalofEcology(生态学杂志), 2009, 28(3):411-416.
[ 14] LIUGY, PENGLJ, CHENFJ.Analysisofamylaseandsuperox-
idedismutaseisozymesduringthegerminationprocessofEmm-
enopteryshenryiolivseeds[ J] .AgriculturalScience&Technolo-
gy, 2009, 10(2):6-8, 19.
[ 15] HUANGLD(黄丽丹), CHEJC(陈剑成), LIJF(李建凡), etal.StudyonseedqualityandseedlinggrowthofMicheliamacclurei
fromdiferentprovenances(不同种源火力楠种子品质和幼苗生长
研究)[J].JournalofAnhuiAgriculturalSciences(安徽农业科学),
2010, 38(34):19422-19425.
[ 16] MENGSC, ZHANGHY, LIUPY, etal.Efectsofultra-drying
treatmentonlargeamountsoffourkindsofcropseeds[J].Agri-
culturalScience&Technology, 2009, 10(2):82-85, 89.
Responsibleeditor:YINQing-qing Responsibleproofreader:WUXiao-yan
(下转第 58页)
51YUJunetal.ResearchontheMorphologicalCharacterandGerminationCharacteristicsofTamarixelongataLedeb.Seeds
efectwasrepresented.Itfoundthatthecompositionofpro-
teinmatrixhadthepositioncorrelationwiththedispersedde-
greeofstarchgrain.Whentheproteinwasdecomposedsufi-
ciently, thedispersingofstarchgrainwasgood.Inthecorn
whosesoakingefectwasgood, thestarchgaindispersed
wel.Thethickproteinmatrixbecamethin, andmostscat-
teredintothesmalfragmentswhichdissociatedinthegapof
starchgrainandthesoakingsolution.Theanalysisprovideda
kindofdirectmeanformonitoringthecornsoakingprocess.
Thetestshowedthatasthesoakingtimeprolongedin60 h,
thecompositionofproteinmatrixwasmoreandmoresufi-
cient.Butconsideringfromtheenergyloss, wesuggestedu-
sing48 hsoaking.
References
[ 1] JIAYT(贾玉涛), LANHZ(蓝海洲), HOUHX(侯汉学).Studyon
laboratory-scalecornstarchwet-milingprocedures(玉米淀粉实验室
提取方法研究)[ J] .JournalofCereals&Oils(粮食与油脂), 2006
(7):29-30.
[ 2] DOWDMK.Improvementstolaboratory-scalemaizewet-miling
procedures[J] .IndustrialCropsandProducts, 2003, 18:67-76.
[ 3] ECKHOFFSR, SINGHSK, ZEHRBE, etal.A100-glaboratory
cornwet-milingprocedure[ J].CerealChem, l996, 73(1):54-
57.
[ 4] PENGGL(彭桂兰).Studyonsteepingprinciples, processandin-
teligenceexpertsystemforcornwetmiling(玉米淀粉湿法加工浸泡
机理、工艺及智能专家系统的研究)[D].Changchun:JilinUniversity
(长春:吉林大学), 2006.
[ 5] DUANYQ(段玉权), LIXH(李新华), MAQJ(马秋娟).Theefectofcelulaseonsteepingtechnologyincornstarchwetmiling
process(纤维素酶对玉米淀粉生产中浸泡效果的影响)[J].Science
andTechnologyofCereals, OilsandFoods(粮食食品科技), 2004,
12(1):14-15.
[ 6] ZHAOSJ(赵寿经), QIANYC(钱延春), LIANGYL(梁彦龙), etal.
Alactobacilusthermophilusselectedandapplyinimprovingprepa-
rationprocessofcornstarch(嗜热乳酸菌的筛选及其在玉米淀粉湿
法生产浸泡工艺中的应用)[J] .FoodandFermentationIndustries
(食品与发酵工业), 2008, 34(1):46-49.
[ 7] RENHS(任海松), DONGHZ(董海洲), HOUHX(侯汉学).Re-searchprogressofreducingsoakingtimeincornwet-milingpro-
duction(玉米湿磨生产中减少浸泡时间的研究进展)[ J].Foodand
NutritioninChina(中国粮食与营养), 2007(5):36-38.
[ 8] LIY(李艳), CHANGJR(常俊然).Researchprogressofcorn
steepingtechnology(玉米浸泡工艺研究进展)[J] .Cereal&Feed
Industry(粮食与饲料工业), 2006(9):25-26.
[ 9] LIUYW(刘亚伟).Starchsproductionanditsprocessingtechnolo-
gy(淀粉生产及其深加工技术)[ M] .Beijing:ChinaLightIndustry
Press(北京:中国轻工业出版社), 2001:1-142.
[ 10] GANFQ, ZHANGYP, YANGQF, etal.Researchonlawsof
starchaccumulationinmicro-endospermmaizekernel[ J].Agri-
culturalScience&Technology, 2009, 10(3):84-87.
[ 11] TANGHM, TANGWG, XIAOXQ, etal.Efectofsuperabsorb-
entpolymersonyieldandwater-savinganddrought-escaping
mechanisminspringmaize[J].AgriculturalScience&Technolo-
gy, 2009, 10(4):112-116.
Responsibleeditor:SONGPing Responsibleproofreader:WUXiao-yan
玉米湿法实验室浸泡工艺的浸泡效果分析
尚会建* ,允川 ,蒋梁鹤 ,王亮 ,杨立彦 ,郑学明 ,王丽梅 (河北科技大学化学与制药工程学院 ,河北石家庄 050000)
[目的 ]确定实验室玉米淀粉生产的最适宜条件 ,并对浸泡效果进行分析。
[方法 ]应用正交试验确定适宜的工艺条件 ,通过光学显微镜和扫描电子显微镜对浸泡效果进行分析。
[结果 ]适宜的浸泡条件为:浸泡时间 48h,浸泡温度 55℃, SO2浓度 0.2%。显微分析结果为:在适宜的浸泡条件下 ,蛋白质基质分解充分。浸泡效果的好坏与蛋白质基质的分解程度呈正相关。
[结论 ]该研究可为实验室玉米淀粉的浸泡工艺研究提供基础。
关键词 玉米;浸泡工艺;显微分析;蛋白质分解
作者简介 尚会建(1972-),男 ,河北石家庄人 ,副教授 ,硕士 ,研究生导师 ,从事传质与分离研究。 *通讯作者。
收稿日期  2010-12-03  修回日期  2011-01-07
(上接第 51页)
长穗柽柳种子形态特征与萌发特性初步研究(摘要)
于军 1 ,姜喜2 ,白宝伟3 ,焦培培4, 5 ,庞新安 4, 5* (1.塔里木大学科技处 ,新疆阿拉尔 843300;2.塔里木大学植物科技学院 ,新疆阿
拉尔 843300;3.塔里木大学生命科学学院,新疆阿拉尔 843300;4.新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室 -省部共建国家重
点实验室培育基地,新疆阿拉尔 843300;5.塔里木大学生物技术研究开发中心 , 新疆阿拉尔 843300)
[目的 ]了解柽柳植物在自然条件下的萌发行为。
[方法 ]以长穗柽柳为实验材料 ,观察研究了种子形态与萌发特性
[结果] ①长穗柽柳种子平均的长、宽、千粒重分别为 1 011.96μm、399.94μm、38.66mg;②自然条件下长穗柽柳种子平均的发芽率 、发芽势、
发芽指数、发芽系数分别达到 95.56%、46.67%、20.25、56.86;③各地理居群之间的种子形态及萌发特性没有显著性差异。
[结论 ]该研究以期为长穗柽柳的引种繁育及沙丘植被保护提供参考。
关键词 长穗柽柳;种子;形态;萌发
基金项目 国家自然科学基金资助项目(30670133)。
作者简介 于军(1968-),男 ,山东乳山人 ,硕士 ,副教授 ,从事荒漠化防治与生态恢复研究。*通讯作者。
收稿日期  2010-11-08  修回日期  2010-12-19
58 AgriculturalScience&TechnologyVol.11, No.11-12, 2010