Abstract:To get effective enzyme hydrolyze cutin of Eucommia ulmoides corneous layer, the extraction of the biological active compositions within Eucommia ulmoides was done using enzymatic hydrolysis. This article from the lesion Eucommia leaves isolated bacteria capable of producing cutinase and identify the bacteria, confirmed that the strain belongs to Rhodotorula mucilaginosa, and the strain producing cutinase fermentation conditions were optimized. This finding is significant, because the yeast more secure than plant pathogens is more suitable for mass production.