摘 要 ：对13株来源于青藏高原土壤的放线菌进行活化，再分别采用琼脂扩散法、生长速率法、孢子萌发抑制法和离体叶片法筛选出一株抗菌活性高且抗性稳定的菌株AL-04。抗菌活性结果表明，该菌株对4种常见的土传病害病原真菌：辣椒疫霉病菌(Phytophthora capsici)、黄瓜枯萎病菌(Fusarium oxysporum f. sp. cucumerinum)、西瓜枯萎病菌(Fusarium oxysporum f. sp. niveum)、番茄灰霉病菌(Botrytis cinerea)都有较强的抑制效果，抑菌率均在70.0%以上，其中对辣椒疫霉病菌(Phytophthora capsici)抑菌率高达93.0%。通过形态特征、生理生化特征分析和16S rDNA序列分析，将该菌株鉴定为杀真菌素链霉菌(Streptomyces fungicidicus)。
Abstract:13 actinomycetes strains were screened from soil samples of Qinghai-Tibet Plateau. Strain AL-04 which has high and stable inhibition effects on soilborne pathogens was selected by methods of agar diffusion, relative growth rate, spore germination inhibition and vegetable leaves. Results of antagonistic activity showed that AL-04 had strong antagonistic activity against 4 species of soilborne pathogens including Phytophthora capsici, Fusarium oxysporum f. sp. cucumerinum, Fusarium oxysporum f. sp. niveum and Botrytis cinerea. The inhibition rates were beyond 70.0% for all pathogens, of which 93.0% was observed for Phytophthora capsici. AL-04 was identified as Streptomyces fungicidicus by the observation of the morphological features, cytochemical characters and 16S rDNA sequence.