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作 者 ：邓馨 胡文玉

期 刊 ：植物学通报 2000年 17卷 02期 页码：174-178

关键词：草莓(Fragaria×ananassa Duch.)；再生芽；转基因；

摘 要 ：本文从五个草莓品种中筛选了再生频率高的品种M14,并进一步提高其再生频率至100%,单叶盘再生芽数至10.0个;石蜡切片法观察其芽多起源于愈伤组织,少数由叶细胞脱分化后直接形成; 该再生体系用于根癌农杆菌菌株EHA105介导的基因转化，并以14.5%频率得到抗卡那霉素的抗性芽,初步鉴定是转化芽。上述结果表明高频率稳定的草莓品种M14 叶片再生芽及遗传转化系统已经形成，并可以用于转基因研究。

Abstract:An ideal regeneration system from leaf of strawberry cultivar M14 was developed. 10.0 shoots per leaf disc were generated at the rate of 100% within 3 weeks. Histological observations indicated that the shoots regenerated mainly from calli. This regeneration system was used in genetic transformation mediated by Agrobacterium tumefaciens strain (EHA105) carrying the modified pMDG410 plasmid, which contains GUS and NPTⅡgene. Kanamycin_resistant shoots regenerated with the ratio of 14.5% were identified as transgenic shoots.

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