Abstract:A new method for sample location in semi_thin sectioning is introduced. This method differs from previous methods in the way of separating the re_embedded section from the glass slide. The slide with polymerized section is taken out from the oven and immediately put in the freezing chamber of a refrigerator and left there for 5-10 mins. Then take the slide out of the freezing chamber and push the plastic ring on the slide. Thus the section in the plastic ring is easily separated from the slide. This method eliminates the danger of losing the sample while the developmental stage of the target cells is being derermined, and has the advantages of simple operation and high rate of success.