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作 者 ：谷瑞升 刘群录 陈雪梅 蒋湘宁

期 刊 ：植物学通报 1999年 16卷 02期 页码：171-177

关键词：木本植物；蛋白提取；十二烷基磺酸钠聚丙烯酰胺凝胶电泳；

摘 要 ：在几种木本植物上对4种常用蛋白提取方法、4种凝胶染色和脱色方法及影响蛋白定量和SDS-PAGE电泳的因素进行了比较研究，发现：利用改良丙酮沉降法提取蛋白，不仅提取效率高，而且杂质干扰少，得到的电泳结果，蛋白条带清晰，数量多；将常规凝胶染色和脱色液中的乙醇改用为甲醇可大大改善染色和脱色效果，获得了没有背景或背景很浅的电泳结果。通过研究确定了一套优化的适用于木本植物的蛋白提取、定量、凝胶制备、电泳、染色、脱色以及干胶制备的方法。利用这一优化方法对胡杨细胞盐胁迫蛋白进行了研究，发现了与高水平盐胁迫有关的66kD蛋白和与盐胁迫和干旱胁迫均有关的28kD蛋白，获得了满意的蛋白SDS-PAGE分析结果。

Abstract:The methods of protein extraction and SDS-PAGE were compared and optimized in woody plants. The improved steps included modified protein extraction, substitution of ethanol for methanol in the solution of staining and destaining. Satisfied results were achieved in the application of the optimized method to the protein analysis of several woody plants. 66 kD protein band related to salt stress and 28 kD protein band associated with osmotic stress were obviously showed out in the analysis for the suspensivly cultured cells stressed with NaCl of Populus euphratica.

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