---

作 者 ：张忠恒 郑易之 段晓刚 佟德娟

期 刊 ：植物学通报 1998年 15卷 增刊期 页码：116-118

关键词：戊二醛；蛋白A-胶体金；免疫电镜标记；

摘 要 ：经4％多聚甲醛+0或0.5％戊二醛固定(1小时)大豆子叶细胞，大部分细胞器和蛋白体发生较大程度的变形，甚至解体。随着固定剂中戊二醛浓度升高至1.5、2.5％，细胞超微结构保存效果提高，蛋白体内反映7S蛋白质存在的胶体金颗粒密度分别为19.7和11.4个／µm2。

Abstract:Most organelles and protein bodies swelled to a large extent, or were ever destroyed in the soybean cotyledon cells fixed with fixatives of 4% formaldehyde + 0,0.5 % glutaraldehyde. But the glutaraldehyde fixation with 1.5, 2.5 % formaldehyde were used as a compromise between structural and antigenic preservation, resulting in the labeling density of 19.7 and 11.4/µm2 of colloidal gold particles on the surface of protein matrix in the protein bodies, respectively.

---