By chromosome C-banding and bi-color fluorescence in situ hybridization (FISH) using digoxigenin-labelled total genomic DNA of Leymus racemosus (Lam.) Tzvel. and biotinylated total genomic DNA of Haynaldia villosa (L.) Schur as probes, three wheat-alien lines with L. racemosus Lr.7 addition and H. villosa 6VS/6AL translocated chromosomes, and eight lines with L. racemosus Lr.14 addition and H.villosa 6VS/6AL translocated chromosomes were respectively identified from DALr.7×T6VS/6AL (93G51-4×P64) and DALr.14×T6VS/6AL （94G15×P64）F2 or F3 hybrids. Fluorescein-isothiocyanate-conjugated avidin and rhodamine-conjugated sheep anti-digoxigenin Fab fragment were used in bi-color FISH detection. The chromosomes of L.racemosus and 6VS fragment of H. villosa were simultaneously detected by their red and green fluorescence. Powdery mildew and scab resistance were also evaluated. The result showed that the obtained plants had high resistance to these two diseases. The potential usage of bi-color FISH in identifying chromatin of L.racemosus and H.villosa was discussed.
普通小麦-大赖草-簇毛麦异附加、易位系的选育和鉴定
陈发棣 陈佩度* 王苏玲
（南京农业大学农业部作物细胞遗传重点开放实验室，南京210095）
关键词： 小麦；大赖草；簇毛麦；易位系；异附加系；双色荧光原位杂交；白粉病抗性；赤霉病抗性
全 文 ：