小麦面粉黄色度b*值是反映面粉颜色的重要指标，主要与籽粒黄色素含量有关。利用122对SSR引物、4对贮藏蛋白STS引物和10对AFLP引物组合，分析了中优9507´CA9632的71个DH系，构建了由173个位点组成的遗传连锁图，在小麦21个连锁群上覆盖2 881 cM。将该群体种植2年共计5个地点，测定籽粒黄色素和面粉黄色度b*值含量。采用复合区间作图法（CIM）进行了籽粒黄色素含量和面粉黄色度QTL分析。结果表明，面粉黄色度b*值的QTL位于染色体1DS、2DL、3A、4D、5D、6AL、6D和7AL上，其中7AL的QTL效应最大，贡献率为12.9%~37.6%；籽粒黄色素含量的QTL位于染色体2DL、3DL、4A、5A和7AL，其中7AL的QTL效应最大，贡献率为12.1%~33.9%。面粉黄色度b*值与籽粒黄色素含量共同的QTL位于7AL，与Xgwm264b紧密连锁，遗传距离分别为0~3.9 cM和0~0.9 cM。
Flour yellowness b* is a major criterion to estimate flour colour, which is basically determined by yellow pigment content in wheat grains. A doubled haploid (DH) population with 71 lines derived from a cross between Zhongyou 9507 with lower flour yellowness b* and CA9632 with higher flour yellowness b*, was sown for two years in 5 locations. A total of 173 loci including 122 SSR, 4 STS of storage protein genes and 10 AFLP markers were used to construct a linkage map, covering 2 881 cM of whole genome at 21 linkage groups. With the method of composite interval mapping (CIM), 8 putative major QTL of yellowness b* were detected on chromosomes 1DS, 2DL, 3A, 4D, 5D, 6AL, 6D and 7AL (Table 2), and 5 putative QTL of yellow pigment content were detected on chromosome 2DL, 3DL, 4A, 5A and 7AL (Table 3), respectively. The QTL on 7AL was common for yellowness b* and yellow pigment content, accounting for 12.9%–37.6% and 12.1%–33.9% of the phenotypic variance across different environments, respectively, which was closely linked to Xgwm264b. The genetic distance between Xgwm264b and the QTL of flour yellowness b* and grain yellow pigment on chromosome 7AL were 0–3.9 cM and 0–0.9 cM, respectively. It can be used as a molecular marker in wheat breeding program.
全 文 ：