The growth of etiolated wheat ( Triticum aestivum L. cv. Lumai No.22) seedlings and the activation of the cell cycle in embryo cells were estimated by flow cytometric analyses in wheat after the seeds being treated with oligoglucosamine and deoxynivalenol (DON). The results indicated that both the root number in etiolated wheat seedlings and the activation of the cells which had been arrested at G1 phase of the cell cycle in wheat embryos were enhanced by oligoglucosamine, suggesting that the mitosis in wheat embryo cells could be promoted by oligoglucosamine. The inhibition of DON on the growth of etiolated wheat seedlings and on the activation of the cell cycle in wheat embryo cells were relieved when the seeds were immersed in oligoglucosamine solution for 12 h before DON treatment. The results indicated that oligoglucosamine increased the hardiness to the poisoning of DON in wheat embryo cells. This might be the reason why such oligosaccharide elicits the resistance of plants to pathogen infection.
壳寡糖诱导小麦种胚细胞抗脱氧雪腐镰刀菌
烯醇抑制的效应
刘晓* 杜昱光** 白雪芳
（中国科学院大连化学物理研究所，大连116023）
摘要：用壳寡糖和脱氧雪腐镰刀菌烯醇(DON)以不同方式处理小麦（Triticum aestivum L.）种子，测定从G 1期启动进入S期和G 2-M期的胚细胞百分率和小麦黄化苗的生长。结果表明：壳寡糖可促进小麦种胚细胞周期启动并促进小麦根数目增加，说明壳寡糖对小麦种子的胚细胞分裂有促进作用；壳寡糖预处理小麦种子可解除DON对小麦黄化苗生长及胚细胞启动的抑制作用，表明寡聚糖可提高植物对病原菌毒素的抗耐性，这可能是寡聚糖诱导植物提高抗病性的重要机制之一。
关键词： 壳寡糖；脱氧雪腐镰刀菌烯醇（DON）；小麦；细胞周期
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