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期 刊 ：植物保护学报 2008年

关键词：小麦赤霉病；黄色镰刀菌；DON毒素；分子定量；

Keywords:Fusarium head blight of wheat, Fusarium culmorum, deoxynivalenol (DON) toxin, molecular quantification,

摘 要 ：为阐明小麦赤霉病穗组织中黄色镰刀菌Fusarium culmorum(简称Fc)与毒素产生之间的关系,分别用TaqMan探针实时定量PCR和ELISA法,对接种4天后的17个小麦品系穗组织中的Fc DNA和脱氧雪腐镰刀菌烯醇(DON)含量进行了测定。在Fc DNA定量检测中,TaqMan探针实时PCR技术具有高度的稳定性和可重复性。不同小麦品系的Fc DNA含量差异显著,感病品种比抗病品种高100倍左右;DON含量也不相同,Fc生物量的增加伴随着DON产量的提高,两者之间存在对数关系(y=19758lnx-111230)。菌量较低时,DON含量相对较大;而菌量较高时,DON含量相对偏低。

Abstract:To elucidate the relationship between fungal biomass and deoxynivalenol (DON) production of Fusarium culmorum (Fc) in Fc-inoculated wheat heads, real-time quantitative polymerase chain reaction (RT-Q-PCR) based on TaqMan technology and enzyme-linked immunosorbent assay (ELISA) were used for Fc DNA quantification and toxin measurement in the head tissues of 17 wheat lines or genotypes 4 days after fungal inoculation. TaqMan-based RT-Q-PCR assay was found to be highly reliable and reproducible to measure Fc DNA. Fc DNA and DON concentrations varied in different wheat varieties or genotypes. A positive and logarithm correlation was observed between Fc biomass and DON concentrations in the Fc-inoculated wheat heads (y=19758lnx-111230).

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