Plant Development from Isolated Microspores of Oryza sativa L. ssp. indica Derived from the Anther Culture of F2 Hybrids of Photoperiod-Sensitive Male-Sterile Rice
Abstract:Microspores from a highly anther culturable rice line (Oryza sativa L. spp. indica) derived from the anther culture of F2 hybrids of photoperiod-sensitive male-sterile rice, after 7 days of low temperature treatment and another 7 days of preculture within anthers, were isolated mmechanically. They were cultured in Ne medium containing 3% manitol, 6% sucrose, 5 g/L inositol, 100 mg/L serine, 800 mg/L glutamine, 1 000 mg/L L-proline, 10% (V/V) coconut milk and 2 mg/L, 2,4-D, and 1 mg/L kinetin. After 5 days, microspores initiated first division and subsequently developed into multicellular pollens and calli. Green plant could be recovered when compact calli were transferred onto agar-solidified MS medium containing 3% sucrose, 0.5 mg/L kinetin, 2 mg/L 6-BA and 1 mg/L IAA.