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Distribution of the cytoplasmic DNA in male cells of the Convolvulaceae and its systematic significance


The distribution of cytoplasmic DNA in generative and sperm cells of four species in four
genera in the Convolvulaceae, i. e. , Calonyction aculeatum , Ipomoea aquatica , Quamoclit pennata
and Cuscuta japonica, has been detected using DAPI fluorescent staining method. Cytoplasmic DNA
is found to occur in generative and sperm cells of all the species except for Cuscuta japonica. This
result, together with the previous reports that cytoplasmic DNA is also distributed in male cells of
five species in Ipomoea, Pharbitis and Calystegia, may indicate that the presence of cytoplasmic
DNA in generative and sperm cells is a general feature of the Convolvulaceae. The absence of such
DNA in male cells in the genus Cuscuta supports the establishment of this genus as an independent
family, the Cuscutaceae.


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