Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA-文献传递-植物通论文库

作者：ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YAN Ji, JIA Ji-Zeng

期刊：植物分类学报 1999年 5期

Abstract:

Ten species of Elymus (Poaceae: Triticeae) were analyzed using random ampli-

lied polymorphic DNA (RAPD) markers. Thirty-four decamer oligonucleotide random

primers from Opron Technologies were used for polymorphic selection. 25 (73.53%) pro-

duced polymorphic products. A total of 136 bands amplified from 16 primers were selected

for RAPD analyses. The data were used to generate Nei‘s similarity coefficients and to con-

struct a dendrogram using UPGMA in NTSYS programs. The result showed that: (1)

Three tetraploid species, E. sibiricus L., E. caninus (L.) L. and E. lanceolatus (Scrib-

ner et Smith) Gould, were clustered in one group, while seven hexaploid species, E. nu-

tans Griseb., E. dahuricusTurcz., E. brachyaristatus A Löve, E. submuticus (Keng)

Keng f., E. tangutorum (Nevski)Hand.-Mazz., E. excelsus Turcz. and E. cylindricus

(Franch) Honda, were clustered in another group. The relationship between the tetraploid

and the hexaploid species was remote; (2) E. caninus was closely related to E. sibiricus.

This result supported that Roegneria canina (L.) Nevski was treated as Elymus caninus;

(3) E . nutans was closely related to E. dahuricus ; (4) E.brachyaristatus and E.sub-

muticus were morphologically similar and sympatric in distribution, but there were certain

differences between them in the nucleotide sequences. Both of them were related to E. nu-

tans and E. duhuricus; (5) E. excelsus was closely related to E. cylindricus, and they

were clustered with E. tangutorum; (6) RAPD results are basically comparable with those

obtained from studies on morphology and cytology. It is a useful supplementary method for

assessing the genetic relationships among Elymus species.

Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA