Abstract:The Giemsa C-banding analyses of type species of Elymus L., Roegneria C. Koch and Hys-trix Moench in Triticeae were carried out by using an improved C-banding procedure. The Giemsa C-banding patterns among E. sibiricus R.caucasica and H. patula were distinct, indicating the interspecific differentiation of the three type species. Most of the Giemsa C-banding diferences of St,H or Y genome in E. sibiricus, R.caucasica and H.patula between other species were discussed.