全 文 ：Chinese Bulletin of Life Sciences
17 2
2005 4
Vol. 17, No. 2
Apr., 2005
1 CA 92521
2 200032
S432.1 A
Nitric oxide and elicitor-induced plant defense responses
HU Xiang-Yang1, CAI Wei-Ming2*
(1 Department of Botany and Plant Sciences, University of California River-side, CA 92521, USA;
2 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,
Shanghai 200032, China)
Abstract: Elicitors, derived from cell wall of fungi or plants, can induce a line of defense responses in plants. During
these processes, a multiple of plant signal molecules, such as nitric oxide, reactive oxygen species (ROS),
jasmonic acid, salicylic acid, ethylene etc., play the essential role in conveying the elicitor stimulation to
intracellular defenses responses. In this review, at first we described the classification of elicitors, and intro-
duced briefly the receptor, conveying and responses of plant cell to elicitor stimulation. At last we focused on
the detailed role and new research proceeding of nitric oxide involving elicitor-induced defense responses, and
its interaction with other plant signal molecules.
Key words: nitric oxide; elicitor; plant defense; signal transduction
1004-0374(2005)02-0176-07
2004-05-26 2004-11-29
(39870050)
( 1 9 7 3 ) ( 1 9 6 2 ) *
( h y p e r s e n s i t i v e
responses, HR)
(
)
(systemic acquired responses, SAR)
(elicitor)
(1981)
(endogenous elicitor);
(genuine elicitor)
[1~2]
1
1.1
[3] OGA
( )
[4]
[3]
1.2 1968
Cruickshank Perrin (Mynilinia fructicola)
(monilicolin A)
(phaseollin)
[1]
hepta-β-glucoside-
alditol 10 3~10 4 µmol/L
0.6 ng
(Cladosporium fulvum) AVR9
CF9 HR
(LPS) (EPS CPS)
Harpin
(Eruinia amylovora)
44kD
hrpN hrp
TMV
PVX N
Rx
( )
1.3
2 -
TMV
50 40
(Cladosporium cucumerium)
1.4
178
DNA
2
G
[5~7]
(HRGP)
( N O )
NO
NO
( 1 )
2.1 NO NO
NOS( nitric oxide sythase) L-
NADPH FAD
FMN NOS
NOS
NOS
NOS
NOS iNOS
NOS 130 kD
NOS
[8~9]
NOS NOS
[10] NOS
NO Yamasaki [11] pH 7.0 NADPH
NO2- NO3 NO NO2- NO
NO3
NO2 NO[12]
iNOS iNOS
NO ABA
NO T DNA
Atnos1 ABA NO
[13] NOS
NOS NOS
NOS
Berthke [14] NO
N O
AHb1(hemoglobin AHb1)
S-
AHb1
[15]
NO NO
2.2 NO NO
1996 , NO
NO
[ 1 6 ~ 1 7 ]
NO 1-Hydro-2-oxo-3, 3(2-aminoethyl)-1-
triazene( NOC-18)
NO PBITU NOC-18
1 NO
CA: ; cADPR: ADP ; C4H: 4
CHS: ; cGMP: GMP; GSNO: S
-L- ; JA : ; MARKs:
; NOS: ; OONO-: ; PAL:
; PHE: ; PR-1: -1;
GST: S ; GPX: ; SA:
; SAR: ; SOD:
NO SNP(
) SNP
[18~19]
SNP
DNA [18]
NO
NO
[9]
(lipopolysaccharides LPS)
LPS
NOS
Pseudomonas syringae pv. tomato DC3000
NOS AtNOS1 [20]
NO
SNP
NO
SNP
cPTIO(NO
) [9]
NOS
[ 9 ]
(Nicotiana tabacum cv. Xanthi NC)
(tobacco mosaic virus Nicotiana tabacum cv TMV)
32 TMV
PR ( )
TMV
2~3
NOS 4~5 NOS
L-NMMA DPI TMV
NOS [19,21~22]
DAF-2-DA(NO ) NO
DAF-2-DA
DAF-2 NO
NO
DAF-2
DAF-2
NO Nakatsubo [23] Foissner
[24]
NO NO
NO
NOS
NO
2.3 NO
NO
Noritake [25] NO
NO NO cPTIO
Triton
NO
NO
Caro Puntarulo[26]
NO NO
(half maximal effect) 0.3 3.6
µmol/L
NO Laxalt [18]
2,4- ( )
SHAM(
) NO
NO
PAL
L-NAA
PAL SNP
PAL [9]
cPTIO NOS
PAL CHS
[21] Delledonne [9]
180
CHS NO cPTIO
(sodium nitro prosside, SNP) CHS
NOS
PR-1 NOS L-NAME NO
cPTIO NOS PR-1
NO S-nitroso-L-glutathione GSNO
SNP PR-1
SA
NOS SA
18 h 2 µg/g
(0.01 µg/gFW)
nahG( )
SA
PR-1 NOS PR-1
NOS nahG PR1
NO PR-1 SA
NOS PAL
nahG NOS PAL
NO PAL
SA [21~23,27]
(nitric oxide dioxygenase NOD)
NOD
NO
NOD
[28]
NO
NO mRNA
mRNA mRNA
mRNA
NO
[29~30] (GST)
GST SNP GST
NO GST
[9] 1 NO
NO
NO
2.4 NO
(MAPK)
NO SA [31]
NO
cGMP [17] NO GSNO
PAL
LY835839(6-anilino-5,8-quinolinedinone)
1H-(1,2,4)-oxadiazole[4,3-a]quinoxalin-1-one(ODQ)
GSNO PAL 8-Br-
c-GMP cGMP
LY835839 GSNO PAL
NO NO
cGMP PAL [32]
NO
NO
NO
cGMP
NO [33]
NO
[34] NO
cGMP N A D ADP-
(cADPR) cADPR
[35] cADPR
cADPR cADPR PR-1
PAL
nahG cADPR
PAL PR-1 cADPR
PR-1 PAL
[21~22] NO
NO
NO
NO
[36] ABA
NO
[37] ABA NR
NO[38] GA
NO GA
[39] KT NO [40]
NO [41]
NO
[34] NO
NO
nox1(
NO) NO
nos1 NO
CONSTANS GIGANTEA
FLOWERING LOCUS C
[42]
3
NO
NOS NO
[43]
NO
NO
N O
NO
NO
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