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 Growth characteristics of phosphorus accumulating organism JPA1 strain and optimization of phosphorus accumulation condition

聚磷菌JPA1菌株的生长特性和聚磷条件的优化



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ChineseJournalofBioprocessEngineering
Vol.10No.2
Mar.2012
doi:10.3969/j.issn.1672-3678.2012.02.007
NOPH
:2011-09-20
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GrowthcharacteristicsofphosphorusaccumulatingorganismJPA1strainand
optimizationofphosphorusaccumulationcondition
XUYe1,2,HUANGZhihua1,GUOHongfang1
(1ColegeofChemicalandBiologicalEngineering,SanmingUniversity,Sanming365004,China;
2ColegeofAgronomyandBiotechnology,YunnanAgriculturalUniversity,Kunming650201,China)
Abstract:BasedontheYGmedia,efectsofdiferentconditionstothegrowthandphosphorusaccumula
tionrateofJPA1strainwerediscussed.ResultshowedthatthelogarithmicphaseofJPA1wasfrom3hto
9h,itturnedintostationaryphaseafter9h,andthenenteredthestateofdeathphase.Singlefactorex
perimentshowedthatmaltosewastheoptimalcarbonsourceforJPA1.Theoptimalgrowthtemperature
was30℃ to35℃.ThebestpHwas8.MetalionsofNa+,Ca2+,Mg2+,Mn2+,andK+werebenefical
tothegrowthofJPA1.Theoptimalphosphorusaccumulationtemperaturewasamong30℃ to35℃,op
timalphosphorusaccumulationpHwas7,MetalionsofFe2+,Na+,Mg2+,Mn2+,andK+weregoodfor
phosphorusaccumulation.BasedontheYGmedia,usingmaltosetoreplaceglucose,orthogonalexperi
mentresultsshowedthatthebestphosphorusaccumulationconditionwasmaltose15g/L,37℃,pH
65,volumeofliquid50mL(shakeflaskculturein300mL);thebestconditionofculturewasmaltose
15g/L,37℃,pH65or75,volumeof150mL(shakeflaskculturein300mL).
Keywords:accumulation;phosphorus;optimization
  
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