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Investigation on oil production by Rhodotorula glutinis

粘红酵母在味精废水中发酵生产油脂



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ChineseJournalofBioprocessEngineering
Vol.8No.1
Jan.2010
doi:10.3969/j.issn.1672-3678.2010.01.002
OPQI
:2009-04-07
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:1672-3678(2010)01-0006-05
InvestigationonoilproductionbyRhodotorulaglutinisin
wastewaterfromglutamateprocess
XINGXu,XUEFeiyan,TANTianwei,ZHUYongqiang
(DepartmentofLifeScienceandTechnology,BeijingUniversityofChemicalTechnology,Beijing100029,China)
Abstract:AcompetentoilproducingRhodotorulaglutinisstrainRh8wasobtainedbyusingwastewater
frommonosodiumglutamateproductionasfermentingmediumbydomesticating.Factorsafectingthepro
ductionofbiomassandoilaswelasCODreductionbystrainRh8werestudied.TheRh8grownwelin
thewastewaterdilutedby4timesandatpH55.Theproductionofthebiomassandtheoils,andthe
CODreductionratewereimprovedbyaddingwasteliquidglucose,yeastextracts,KH2PO4,MgSO4,and
MnSO4.Thebiomassproductionwas156g/L,oilproductionratewas2961% ina250mLshaking
flaskandCODreductionratereached45.1%.
Keywords:glutamatewastewater;microbialoil;Rhodotorulaglutinis
  
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8,
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4,
@
20。
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(g/L):
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40,
(NH4)2SO42,KH2PO47,Na2SO42,MgSO4·7H2O
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15。
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monosodiumglutamatewastewater
  
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1 
ÅƳÇÈ}[É_ÃÄ
Table1 Efectsofaddingwasteliquidglucose
ρ(•–p)/
(g·L-1)
ρ(>y?)/
(g·L-1)
?@0r


ρ(Ç?@)/
(g·L-1)
COD
R2r
/%
zYqr
/

0 725 2052 149 2463 —
5 103 2335 241 254 368
10 1316 2631 346 2496 394
20 1324 2456 325 2357 176
30 1407 2631 37 2271 147
40 148 2744 406 2195 129
   
Æ

zYqr

?@?

•–p‹Œ?
×100%。
‚
2 
ÅÆZ[Í_ÃÄ
Table2 Efectsofaddingyeastextracts
ρ(-.G)/
(g·L-1)
ρ(>y?)/
(g·L-1)
?@0r


ρ(Ç?@)/
(g·L-1)
COD
R
2r
/%
05 875 2132 187 1834
1  836 2204 184 1725
15 892 2183 195 1801
2  824 2175 179 1774
2.5.2 
߀À¬3ST
8@eW‘’žŸ=

߀À¬ÍBEøý"Ü
H=Oô.ªH3å‡

Q?@3‘’DJEìv3
ST

ÇfÀK¨
,KH2PO4
[11]、MgSO4、MnSO4
[12]
:
ðQ/N?@3‘’Dj0á

de

¿å8㈌
;<(3H³Á

úĽ=ŒŸs]?3߀À
¬

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¿Ñú;<=‹Œ
2g/LKH2PO4、1g/L
MgSO4、05g/LMnSO4,/-œœKþ 3。Ì 3
Í!

߀À¬3‹ŒQ|>y?ª?@0r3ˆ
ŒðDs]B£

ÙÚ®2³EF
COD
3R2r

R=
,KH2PO4Q COD3ÁR£KTEØ®,S
MgSO4:?@>?³E*2961%,MnSO43:>y
?³EF
352%。
‚
3 
ÅÆCÎÏÌ_ÃÄ
Table3 Theefectsofaddingionson
À¬

ρ/
(g·L-1)
ρ(>y?)/
(g·L-1)
?@0r


ρ(Ç?@)/
(g·L-1)
COD
R
2r


KH2PO4 2 1326 2343 311 451
MgSO4 1 1475 2961 437 392
MnSO4 05 156 2593 405 421

— 1154 2420 279 287
2.6 
¬µ_ÐÑÒÓfw
ÿ

X67-.
Rh8
Nm89:;<=/-
j0?@3uv’¿¿«ÿ

Ìÿ

Í!Ruv’
¿E56W
(C16∶0),
7@W
(C18∶0),
?W
(C18∶
1),
C§ÒDy¿?8

e
4 Rh8
›\¬µ_
GC
fwÓe
Fig.4 GCanalysisofoilsproducedbyRh8
9 
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3 
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ëžÃYABq*sm0?j­Jp367
-.Nm
Rh8,
ðQR¢£E ˜9:;<×n/
->0¯>y?@3{|×nF6t45

œK 
Ø

TE?@>y?)*
156g/L,
?@0r8
30%
MN

ÙÚ
COD
ôirÙ
50%。
If=j£
67-.NmÍ=¿qJE
COD
39:;<

×s
t9Y„¡YžŸ

ÙÚ

ñNmQ9:;<=E
 ˜
(NH4)2SO43àékÛ×stˆp,=:|q
£*É[>0Y=

¢£E ˜9:;Y;<

í;RQ893¢£

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M“6i

[1] 
<=>

½?@

AW

9:;[J].
=
ÙF<™<
,1998,14(6):2325.
BaoQijun,ZhangHongchang,ZengHua.Discussionontreatment
ofmonosodium glutamatewastewater[J].ChinaWater&
Wastewater,1998,14(6):2325.
[2] 
GdH

—I

½?J

:

9:;345
[J].
89¢£BYäåÒzh
,2000,1(6):3540.
YangJianzhou,WenGuan,ZhangHongxun,etal.Studyonfer
mentationpropertiesofBacilusthuringiensisintheglutamate
wastewater[J].TechEquipEnvironPolutionControl,2000,1
(6):3540.
[3] 
×M

G¨N

GO

:

¢£-.N¡YE ˜9:;<3
PQR¥
[J].
89ÝÞ
,2002,23(4):6266.
HeiLiang,YangQingxiang,YangMin,etal.Laboratoryscale
continuoustreatmentofmonosodium glutamatemanufacturing
wastewaterusingyeast[J].EnvironSci,2002,23(4):6266.
[4] 
GdH

½ST

½?J

¢£9:;<>0ªCƒ„34
5
[J].
89¢£BYäåÒzh
,2002,3(6):2124.
YangJianzhou,ZhangSongpeng,ZhangHongxun.Productionof
microbialproteinfeedfrommonosodiumglutamatewastewaterby
fermentation[J].TechEquipEnvironPolutionControl,2002,3
(6):2124.
[5] 
UVk

Œ>¯>y?@45E>y|?0[³;IJ«C
[J].
=Ù>yɟX¾
,2005,25(2):811.
ZhaoZongbao.Towardcheapermicrobialoilforbiodieseloil[J].
ProgBiotech,2005,25(2):811.
[6] 
½¾7

WXY

”•–

67-.¡Y9:;<345
[J].
‰~YÉíÞÞK

ÛÜÝÞL
,2007,34(1):9597.
ZhangZhihong,XueFeiyan,TanTianwei.Treatmentofmonosodi
umglutamatewastewaterbyRhodotorulaglutinis[J].JBeijing
UnivChemTech:NatSci,2007,34(1):9597.
[7] BlighEG,DyerWJ.Arapidmethodoflipidextractionandpuri
fication[J].CanadianJBiochemPhysiol,1959,35:911917.
[8] 
Ú7

Z.[

\dW

<û¿«ùf
[M].
‰~

5]ÏLZ

2001:202204.
[9] 
ƒ^_
,`
a

67-.
GLR513>0?@TòR­ÉÊ/-{
|3DE
[J].
bcÝÞ
,2003,24(1):4851.
ShiAnhui,ZhouBo.Studyonoptimumminitypefermentation
conditionsoflipidproductionbyRhodotoruleGlurtinisGLR513
[J].FoodScience,2003,24(1):4851.
[10]
dUY

efg

hij
.¯
>yijk?@45kA
[J].
l
bÒ?@
,2003(11):1214.
PuHaiyan,HeZhifei,LiuChunfen.Researchsurveyonfunction
alfatsandoilsfrommicroorganisms[J].JCerealsOils,2003
(11):1214.
[11]
mn

o’p

qrJ

:

0?sN8tFG;<=/-3
6t45
[J].
>yäå
,2007,17(2):7275.
DuJuan,ZhanChengxiong,WangHongxun,etal.Elementary
studyonoilproductionbyfungigrowinginsweetpotatostarchpro
cessingwastewater[J].Biotechnology,2007,17(2):7275.
[12]
mn

qrJ

ß7

:

tFG;0¯>y?@
345
[J].
>yŒÉžŸ
,2007,5(1):3336.
DuJuan,WangHongxun,JinHonglin,etal.Fatyacidsproduc
tionbyfungigrowinginsweetpotatostarchprocessingwastewater
[J].ChinJBioprocEng,2007,5(1):
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