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作 者 ：李潞滨,刘振静,杨凯*,刘敏,周金星,孙磊,韩继刚

期 刊 ：生态学报 2008年 28卷 11期 页码：5482~5487

关键词：青藏铁路；唐古拉山口；微生物；多样性；ARDRA；

Keywords:Qinghai-Tibet railway, Tanggula Mountain Pass, microorganisms, population diversity, ARDRA,

摘 要 ：通过构建16S rDNA文库及文库的限制性片段长度多态性分析（ARDRA），对青藏铁路沿线唐古拉山口的土壤微生物多样性进行了研究。采用限制性内切酶HaeIII和RsaI对克隆文库中的90个克隆子进行了酶切分型，根据ARDRA酶切图谱的不同，可将其分为23个OTUs。16S rDNA序列分析结果表明，该克隆文库中主要包括变形菌门（Proteobacteria）的alpha、beta、detla亚类、厚壁菌门（Firmicutes）、放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）、酸杆菌门（Acidobacteria）及浮霉菌门（Planctomycetes）等8类细菌及未培养细菌。Alpha变形细菌为该文库中的主要菌群，占克隆总数的33.3%；其次为未培养细菌，占克隆总数的22.2%，Bradyrhizobium为优势菌属。研究结果揭示，青藏铁路唐古拉山口的土壤微生物种群不仅具有丰富的多样性，还存在丰富的潜在新菌种。

Abstract:The soil microbial diversity of Tanggula Mountain Pass along the Qinghai-Tibet railway was analyzed by 16S rDNA cloning, amplified ribosomal DNA restriction analysis (ARDRA), and sequence homology comparisons. Among 90 positive clones in the 16S rDNA library of microorganisms, 23 OTUs (Operational Taxonomic Units) were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, delta subclasses of the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria and Planctomycetes. The dominant group was alpha-Proteobacteria (33.3% of the total clones), and the most dominant genus was Bradyrhizobium. More than 22.2% of the total clones showed high similarity to uncultured bacteria. The results suggest that the soil microbial diversity is abundant in this area.

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