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作 者 ：颜庆云,余育和,冯伟松

期 刊 ：生态学报 2005年 25卷 9期 页码：2221~2226

关键词：浮游生物群落；RAPD；DNA指纹拓扑结构；生物多样性；洞庭湖；

Keywords:plankton community, RAPD, DNA fingerprinting topological structure, biodiversity, Dongting Lake,

摘 要 ：对东洞庭湖7个采样站点的浮游生物群落进行了DNA多态性的RAPD指纹分析和物种组成的分类鉴定,并通过聚类分析探讨了DNA指纹拓扑结构与物种组成对应关系。结果如下:(1)筛选出的11条随机引物共获得148条长度在180~2000bp的谱带,多态率为98.6%,各引物扩增谱带数在11~16不等;各站点平均有57.6条谱带,其中站最多(70条),站最少(45条),而站的特有带最多(7条),站最少(2条);(2)共观察到54种/类浮游生物,其中站出现的最多(27),其它各站点在7~13不等,分布概率在85%以上的只有直链硅藻(Melosirasp.)。相似性聚类分析表明:7个站点的浮游生物群落可以划分为两大类——站作为单独的一类而明显有别于其它6站;并且,RAPD分析在此基础上将、、站和、、站进一步分作两小类。因此,研究说明浮游生物群落DNA指纹拓扑结构与物种组成是密切相关的,这类资料的积累将会为生态系统功能、机理的解释或阐明提供一些启示。

Abstract:As the confluence of natural resources, biodiversity has become the focus of ecological study. Plankton, as an important part of the biodiversity and cosmopolitan organisms, plays a key role in nutrient cycling and energy flows. Because of its high sensitivity to environmental changes and its short life span, plankton is also used as indicators of environmental variables and aquatic ecosystem health. However, most of the studies have focused on investigating the community composition. To completely reveal t...

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