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作 者 ：张颖娟,杨持

期 刊 ：生态学报 2002年 22卷 11期 页码：1917~1922

关键词：RAPD；四合木；遗传多样性；斑块；基因流；

Keywords:RAPD, Tetraena mongolica, genetic diversity, patch, gene flow, metapopulation,

摘 要 ：采用随机扩增多态性 DNA(RAPD)技术检测了西鄂尔多斯高原特有种四合木种群 5个斑块的遗传多样性。2 2个扩增引物产生 1 1 6条带 ,Shannon信息指数和 Nei指数对 RAPD数据的分析表明 :四合木种群存在较高的遗传多样性 ,其中千里山斑块的遗传多样性和多态位点比例较高 ,石嘴山斑块的最低。遗传多样性的 86.5 %存在于斑块内 ,斑块间的遗传变异为 1 3 .5 %。遗传距离与地理距离无直接相关关系。这些结果说明 ,遗传多样性反映了四合木种群基因组 DNA存在较高的变异性 ;同时各斑块间存在一定的基因流 ,四合木各斑块可看成是处于同一种群的半隔离状态 (meta-种群 ) ,对四合木应注意保护遗传多样性丰富的 meta-种群。

Abstract:Tetraena mongolica Maxim., a monotypic genus of Zygophyllaceae endemic to China, it is an ancient relic shrub and second\|class conserved plant in nation. T.mongolica grows in semi\|arid and arid regions of western ORDS plateau, where T.mongolica plays an important role in soil conservation. However, T.mongolica is affected by a variety of factors.Its distribution is being continually reduced. Its regeneration is difficult and it is increasingly endangered. Random amplified polymorph...

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