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作 者 ：李友国,周俊初

期 刊 ：生态学报 2002年 22卷 9期 页码：1420~1424

关键词：费氏中华根瘤菌；共生质粒消除菌株；大豆根圈；定殖；发光酶基因；

Keywords:Sinorhizobium fredii, pSym curved mutant, soybean rhizosphere, root colonization, luxAB,

摘 要 ：比较研究了费氏中华根瘤菌 (Sinorhizobium fredii) HN0 1 (出发菌 )、发光酶基因标记菌 HN0 1 L(参照菌 )、消除HN0 1共生质粒的菌株 HND2 9SR在无菌砂培条件下的大豆根圈定殖动态。供试菌单独接种时 :HN0 1、HN0 1 L和HND2 9SR的定殖动态基本一致 ,其早期定殖密度下降较快 ,播种后第 1 6天时 HN0 1和 HN0 1 L分别达到较高的定殖水平 6.49logcfu/ g鲜根和 6.78logcfu/ g鲜根 ,然后维持相对稳定的定殖水平。但 HND2 9SR的定殖密度持续下降到播种后第 1 6天时才开始上升 ,至第 3 5天时仍维持相对稳定的定殖密度 6.94logcfu/ g鲜根。等量混合接种时供试菌在根圈定殖群体中各自定殖密度在测定过程中基本相等。结果表明消除 HN0 1的共生质粒对其在大豆根圈中定殖能力无显著影响。

Abstract:Root colonization is a first step during the development of symbiosis between rhizobium and legume plant. It was reported that rhizobium colonization on roots was determined by its ability of chemotaxis and mobility. The relationship between indigenous plasmid and root colonization was reported by using luxAB gene marked strains and modified Leonard jar apparatus. The root colonization dynamics of Sinorhizobium fredii HN01, HN01L marked with luxAB and HND29SR (pSym cured HN01) in rhizosphere of...

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