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作 者 ：陈拓,安黎哲,冯虎元,杨景宏,王勋陵

期 刊 ：生态学报 2001年 21卷 4期 页码：579~583

关键词：UV-B辐射；蚕豆；膜脂过氧化；多胺；

Keywords:UV-B radiation, broad bean, membrane lipid peroxidation, polyamine,

摘 要 ：温室种植的蚕豆在 0 ( CK)、8.82 k J/ m2 ( T1)和 1 2 .6k J/ m2 ( T2 ) 3种剂量的紫外线 B( UV-B)辐射引起膜脂变化及其机制的研究结果表明 ,UV-B处理后 ,蚕豆叶片中丙二醛 ( MDA)和 H2 O2 含量升高 ,膜脂肪酸不饱和度指数 ( IUFA)降低 ,脂氧合酶 ( LOX)活性升高 ,超氧歧化酶 ( SOD)活性稍有波动 ,而 3种多胺——腐胺 ( Put)、精胺 ( Spd)和尸胺 ( Spn)在照射7d后均有积累 ,但在处理后期 ( 2 1 d)有所回落。推测由 LOX主导的酶促膜脂过氧化作用和氧自由基引起的非酶促过氧化作用在膜结构的破坏中起重要作用 ,SOD活性和多胺含量的变化是蚕豆对 UV-B胁迫的一种适应性生理反应。

Abstract:The changes and mechanism of membrane lipids in the broad bean leaves exposed to 0(CK)、8.82kJ/m 2(T 1) and 12.6kJ/m 2(T 2) of UV-B radiation were observed respectively.The results showed that the contents of malonaldehyde (MDA) and H 2O 2 were increased,and that of index of unsaturated fatty acid (IUFA) was decreased,lipoxygenase (LOX) activity was enhanced and superoxide dismutase (SOD) activity was slightly changed after UV-B radiation treatment.The diamine putrescine (Put),the polyamine spermidine ...

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