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作 者 ：操国兴,钟章成,谢德体,刘芸,龙云

期 刊 ：生态学报 2003年 23卷 8期 页码：1583~1589

关键词：RAPD；川鄂连蕊茶；群落类型；DNA多样性；遗传分化；

Keywords:RAPD, Camellia rosthorniana, community types, DNA diversity, genetic differentiation,

摘 要 ：采用随机扩增多态性 DNA( RAPD)对常绿阔叶林灌木层广布种川鄂连蕊茶 ( Camellia rosthorniana)在缙云山 3个群落类型 (毛竹林、针阔混交林和常绿阔叶林 )中的遗传结构和 DNA多样性进行了研究。 1 0个引物共扩增出 1 38个产物 ,其中 1 1 5个是多态性的 ,多态位点比率为 83.33%。Shannon指数估算的 3个种群的遗传多样性为常绿阔叶林 0 .3345 ,毛竹林 0 .31 48,针阔混交林 0 .30 85 ,遗传分化为 8.1 2 % ;Nei指数计算的 3个种群的基因多样性为常绿阔叶林 0 .2 0 95 ,毛竹林 0 .1 981 ,针阔混交林 0 .1 934,遗传分化为7.5 1 %。种群间遗传距离在 0 .0 1 77～ 0 .0 393之间。川鄂连蕊茶在 3个群落中的种群大小和生长状况不同 ,各种群遗传多样性的高低可能是对所处群落生境的适应。

Abstract:With special geographic location and climatic conditions, the subtropical areas in China has developed the largest and typical evergreen broadleaved forest in the world, evergreen shrubs is one important constituent of the forest. Unfortunately, little information is available with regard to the genetic variation of evergreen shrubs in evergreen broadleaved forest. Camellia rosthorniana (Theaceae) is a long-lived evergreen shrub species, which distributes widely in subtropical areas of China and occurs in ...

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