Abstract:Peanut (Arachis hypogaea L.) is an important oil and economic crop in China. Peanut Seed dormancy is an important agronomic trait which can affect quality, yield and seed quality in agricultural production. To dig important functional genes controlling seed dormancy, full-length of auxin-reprssed protein gene was screened from seed of peanut cultivar Huayu52 by RNA-seq, and designated as AhARP in this article. The whole sequence of AhARP was 918bp and its open reading frame was 363bp, encoding a 121 amino acid residues. The deduced protein molecular weight was 13.44KD and its theoretical isoelectric point was 9.64. Sequenc alignment of the deduced amino acids of AhARP revealed high similarity with auxin-repressed protein gene(AAZ20292.1)of peanut. The results of Real-time RT-PCR showed that AhARP gene expressed at a higher level in dormant variety than in non-dormant variety; The expression of AhARP in dormancy-released seed was induced distinctly by ethephon. That indicated AhARP may be correlated to the seed dormancy of peanut. According to the gene sequence and preliminarily predicting structure, nature and functions of AhRP gene. The study have acquired valuable information which can be used to study the biological function of the AhARP gene related to peanut seed dormancy.